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Merck

F8758

Sigma-Aldrich

Folic acid

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥97%

Sinónimos:

Pterylmonoglutamic acid, PteGlu, Pteroyl-L-glutamic acid, Vitamin M

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About This Item

Fórmula empírica (notación de Hill):
C19H19N7O6
Número de CAS:
Peso molecular:
441.40
Beilstein:
100781
Número CE:
Número MDL:
Código UNSPSC:
12352205
eCl@ss:
34058014
ID de la sustancia en PubChem:
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

synthetic

Nivel de calidad

Línea del producto

BioReagent

Ensayo

≥97%

Formulario

powder

mol peso

Mw 441.40 g/mol

técnicas

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

dark yellow

solubilidad

1 M NaOH: soluble 50 mg/mL, clear to very slightly hazy, faintly yellow to dark yellow-orange

temp. de almacenamiento

room temp

cadena SMILES

NC(N1)=NC(C2=C1N=CC(CNC3=CC=C(C(N[C@@H](CCC(O)=O)C(O)=O)=O)C=C3)=N2)=O

InChI

1S/C19H19N7O6/c20-19-25-15-14(17(30)26-19)23-11(8-22-15)7-21-10-3-1-9(2-4-10)16(29)24-12(18(31)32)5-6-13(27)28/h1-4,8,12,21H,5-7H2,(H,24,29)(H,27,28)(H,31,32)(H3,20,22,25,26,30)/t12-/m0/s1

Clave InChI

OVBPIULPVIDEAO-LBPRGKRZSA-N

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Descripción general

Folic acid (FA)/pterylmonoglutamic acid[1] helps to block neural tube defects (NTDs).[2]

Aplicación

Folic acid has been used:
  • to study the role of vitamins and amino acids on hybridoma growth and monoclonal antibody production[3]
  • in cells and treatments[4]
  • in perforin gene targeting using the CRISPR/Cas gene editing approach[5]

Acciones bioquímicas o fisiológicas

A nutritional delivery form of folate. Folic acid and its derivatives are essential mediators of one-carbon metabolism within cells.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Visite la Librería de documentos

Bone marrow derived mesenchymal stem cells ameliorate inflammatory response in an in vitro model of familial hemophagocytic lymphohistiocytosis 2
Sevim H, et al.
Stem Cell Research & Therapy, 9(1), 198-198 (2018)
Folic acid handling by the human gut: implications for food fortification and supplementation
Patanwala I, et al.
American Journal of Clinical Nutrition, 100(2), 593-599 (2014)
Periconceptional folic acid supplementation and the risk of preterm births in China: a large prospective cohort study
Li Z, et al.
International Journal of Epidemiology, 43(4), 1132-1139 (2014)
Reduced expression of galectin-9 contributes to a poor outcome in colon cancer by inhibiting NK cell chemotaxis partially through the Rho/ROCK1 signaling pathway
Wang Y, et al.
PloS one, 11(3), e0152599-e0152599 (2016)
The role of vitamins and amino acids on hybridoma growth and monoclonal antibody production
Ducommun P, et al.
Cytotechnology, 37(2), 65-73 (2001)

Artículos

Explore folic acid importance in serum-free eukaryotic cell cultures, including CHO cells, and its role in media supplementation.

Explore folic acid importance in serum-free eukaryotic cell cultures, including CHO cells, and its role in media supplementation.

Explore folic acid importance in serum-free eukaryotic cell cultures, including CHO cells, and its role in media supplementation.

Explore folic acid importance in serum-free eukaryotic cell cultures, including CHO cells, and its role in media supplementation.

Questions

1–5 of 5 Questions  
  1. Is it better to store folic acid (Product No. F8758) solution at RT or -20'C? Also, how long is it stable as a solution for at recommended temperature?

    1 answer
    1. Solution stability has not been determined for this product, however commercial and pharmaceutical preparations are cited as being stable at room temperature for up to 18 months, protected from light. Folic acid is also a component of most of the classical DMEM media formulations, for example D5546. These products are also assigned a shelf life of 18 months, but are stored at 2 - 8 deg. C. Storing at -20 deg. C may or may not extend the shelf life. There is a possibility that the freeze/thaw process may result in precipitation and may be detrimental.

      Helpful?

  2. What is the stability of folic acid (Product No. F8758) once in solution?

    1 answer
    1. This product is photolabile and is inactivated by UV light. Alkaline solutions are reasonably stable in the dark, but heating will destroy both alkaline and acidic solutions.

      Helpful?

  3. What is the solubility of folic acid (Product No. F8758)?

    1 answer
    1. Product No. F8758 is soluble in 1 M NaOH (50 mg/mL). The free acid is only slightly soluble in water (0.01 mg/mL at 0 °C), and is insoluble in aqueous solutions below pH 5. It is soluble in sodium bicarbonate or sodium hydroxide solutions.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Is Product No. F8758, Folic Acid, tested for endotoxins?

    1 answer
    1. No, Product No. F8758 has not been tested for endotoxins. This product has, however, been tested for use in mammalian cell culture and insect cell culture.

      Helpful?

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