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B3804

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

Sinónimos:

5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Quality Level

100

form

liquid

storage temp.

2-8°C

Categorías relacionadas

General description

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity[1][2] such as western blot analysis.[3]

Caution

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Other Notes

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
MKCV4566
MKCT4192
MKCR6740
MKCR2607
MKCN6094

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Yu A Nashchekina et al.
Bulletin of experimental biology and medicine, 163(1), 123-128 (2017-06-06)
Collagen I gels with protein concentrations of 1, 2, and 3.5 mg/ml were prepared and embedded in a porous polylactide scaffold to reduce their contraction. Concentration of the gel did not affect its degradation. Collagen gels promoted the formation of
Hermann Ehrlich et al.
Nature chemistry, 2(12), 1084-1088 (2010-11-26)
The minerals involved in the formation of metazoan skeletons principally comprise glassy silica, calcium phosphate or carbonate. Because of their ancient heritage, glass sponges (Hexactinellida) may shed light on fundamental questions such as molecular evolution, the unique chemistry and formation
Prafulla Katkar et al.
Metallomics : integrated biometal science, 6(1), 117-125 (2013-11-01)
The ability of oxindolimine copper(II) and zinc(II) complexes, known to have antitumor activity, to inhibit human topoisomerase IB has been tested through enzymatic kinetic assays and molecular docking simulations. These copper and zinc compounds are able to inhibit remarkably the
Stephen Hearty et al.
Journal of microbiological methods, 66(2), 294-312 (2006-02-07)
A panel of hybridomas was produced using intact Listeria monocytogenes serotype 1/2a cells as the immunogen. An IgG2a monoclonal antibody (mAb) 'mAb2B3' was isolated that reacted with L. monocytogenes but not with a representative panel of related Listeria spp. and
Stephen P Christiansen et al.
Investigative ophthalmology & visual science, 47(2), 605-613 (2006-01-25)
A common treatment for motility disorders of the extraocular muscles (EOMs) is a resection procedure in which there is surgical shortening of the muscle. This procedure results in rotation of the globe toward the resected muscle, increased resting tension across
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Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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