11585029001

Nitroazul de tetrazolio

crystals, pkg of 5 g

• Sinónimos: 3,3′-(3,3′-dimetoxi-4,4′-bifenileno)bis[cloruro de 2-(4-nitrofenil)-5-fenil-2H-tetrazolio], Azul de pnitrotetrazolio, Cloruro de p-nitroazul de tetrazolio, Cloruro de 2,2′-bis(4-nitrofenil)-5,5′-difenil-3,3′-(3,3′-dimetoxi-4,4′-difenileno)ditetrazolio, NBT, Nitro BT
• Fórmula lineal: C₄₀H₃₀N₁₀O₆ · 2Cl
• Número de CAS: 298-83-9
• Peso molecular: 817.64
• Beilstein: 4115923
• Número MDL: MFCD00012159
• Código UNSPSC: 41116100
• ID de la sustancia en PubChem: 329749491

Propiedades

• descripción: 4-Nitro blue tetrazolium chloride, crystals
• Nivel de calidad: 100
• Formulario: crystals
• envase: pkg of 5 g
• fabricante / nombre comercial: Roche
• temp. de almacenamiento: 2-8°C
• cadena SMILES: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• Clave InChI: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Descripción

Descripción general

NBT is soluble in water, aqueous buffers, and dimethylformamide.

Tetrazolium salts are useful in determining the metabolic activity of cells from many origins. The quaternary tetrazole ring core containing four nitrogen atoms, with a net positive charge induces cellular uptake through plasma membrane potential. This drives its use in cell biology. In immunolabeling technique, 4-Nitro blue tetrazolium (NBT) serves as an oxidant. The hydrolysis of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) by alkaline phosphatase gives rise to leucoindigo, which is then being reduced by NBT to form an insoluble blue BCI. Certain cells reduce NBT, which is a colorless/yellow dye to blue or black formazan crystals. Due to this property, NBT is prefered in redox histochemistry and in biochemical applications. It is formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Aplicación

• Colony and plaque hybridization
• Immunohistocytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

NBT has been used in in situ hybridization.

Calidad

Performance-controlled in the LDH assay.

Nota de preparación

Working solution: Solubility: soluble in water, aqueous buffers, and dimethylformamide. Can be dissolved in methanol. A clear solution is achieved at 20 mg/ml.

Nota de análisis

Absorption: NBT has its maximum absorption at 260 nm [in water],
and the final product formazan has its maximum absorption at 605 nm [in nitrobenzole].

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302,H315,H319,H335
• Consejos de prudencia: P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233
• Clasificaciones de peligro: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash