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Documentos clave

54327

Sigma-Aldrich

Lipase, immobilized on Immobead 150 from Pseudomonas cepacia

≥900 U/g

Sinónimos:

Immobilized Lipase

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About This Item

Código UNSPSC:
12352204
NACRES:
NA.54

origen biológico

bacterial (Pseudomonas cepacia)

Nivel de calidad

Formulario

powder (or beads)

actividad específica

≥900 U/g

color

white to off-white

aplicaciones

life science and biopharma

temp. de almacenamiento

2-8°C

Información sobre el gen

Burkholderia spp. ... BURCE16_RS29975(56665765)

Descripción general

Research area: Cell Signaling

Lipases are water-soluble, ester hydrolases. Long aliphatic chain acyl esters of cholesterol (cholesteryl esters), triacyl esters of glycerol (triacylglycerols), acyl esters of long chain alcohols (wax esters), diacyl esters of glycerol (diacylglycerols), and monoacyl esters of glycerol, are some of the common substrates for lipases.

Aplicación

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.Lipase, immobilized on Immobead 150 from Pseudomonas cepacia has been used in enzymatic hydrolysis of polylactic acid (PLA). It has also been used as biocatalysts in lipase-catalyzed kinetic resolution reactions.

Acciones bioquímicas o fisiológicas

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.Lipases are known to regulate energy metabolism of the cell. Lipolysis is highly essential for cellular uptake or release of fatty acids (FAs) and glycerol. Gastrointestinal lipolysis catabolizes dietary fat. Vascular lipolysis hydrolyzes lipoprotein-related triglycerides (LTPs) in the blood. In the intracellular domain, lipolysis catalyzes the breakdown of triglycerides (TGs) stored in LDLs for exportation of fatty acids (fat) (fat tissue) or metabolism (non-adipose tissue).

Definición de unidad

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Fatty Acid Signaling: The New Function of Intracellular Lipases
Papackova Z & Cahova N
International Journal of Molecular Sciences, 3831?3855-3831?3855 (2015)
Studies on the chemoenzymatic synthesis of (R)-and (S)-methyl 3-aryl-3-hydroxypropionates: the influence of toluene-pretreatment of lipase preparations on enantioselective transesterifications
Borowiecki P & Bretner M
Tetrahedron Asymmetry (2013)
Florian Golombek et al.
ACS omega, 6(43), 29192-29200 (2021-11-09)
Deracemizations are clearly preferable to kinetic resolutions in the production of chiral molecules from racemates, as they allow up to 100% chemical and optical yield. Here we present a new process route for multienzymatic deracemizations that is relevant for reaction
Rudolf Zechner et al.
Cell metabolism, 15(3), 279-291 (2012-03-13)
Lipolysis is defined as the catabolism of triacylglycerols stored in cellular lipid droplets. Recent discoveries of essential lipolytic enzymes and characterization of numerous regulatory proteins and mechanisms have fundamentally changed our perception of lipolysis and its impact on cellular metabolism.
Lipases
H.L. Brockman
Encyclopedia of Biological Chemistry, 729-732 (2013)

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