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D1435

Sigma-Aldrich

Dimetilsulfóxido

meets EP testing specifications, meets USP testing specifications

Sinónimos:

DMSO

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About This Item

Fórmula lineal:
(CH3)2SO
Número de CAS:
67-68-5
Peso molecular:
78.13
Beilstein:
506008
Número CE:
200-664-3
Número MDL:
MFCD00002089
Código UNSPSC:
12191502
ID de la sustancia en PubChem:
57654071
NACRES:
NA.71
Ensayo:
≥99.5%
técnicas:
cryopreservation: suitable
bp:
189 °C (lit.)
presión de vapor:
0.42 mmHg ( 20 °C)
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Agency

meets EP testing specifications
meets USP testing specifications

Nivel de calidad

200

densidad de vapor

2.7 (vs air)

presión de vapor

0.42 mmHg ( 20 °C)

Ensayo

≥99.5%

Formulario

liquid

temp. de autoignición

573 °F

lim. expl.

42 %, 63 °F

técnicas

cryopreservation: suitable

impurezas

≤0.1% water (Karl Fischer, USP)
≤0.2% water (Karl Fischer, EP)

color

colorless

índice de refracción

n20/D 1.478-1.479
n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubilidad

H2O: miscible (completely)

densidad

1.100-1.104 g/mL at 25 °C (relative density (EP))
1.10 g/mL (lit.)

Absorción UV

λ: 285 nm Amax: ≤0.20
λ: 295 nm Amax: ≤0.20

cadena SMILES

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

Clave InChI

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Aplicación

Dimethyl sulfoxide has been used:
  • for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments[1]
  • as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells[2]
  • for the suspension and separation of buccal cells[3]
  • a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Acciones bioquímicas o fisiológicas

Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant.[4] It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.[5]

Precaución

Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.

Otras notas

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
SHBS3830
SHBR8495
SHBQ2552
SHBS0423
SHBP4255

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Visite la Librería de documentos

Comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood
Chen G, et al.
Stem Cells International, 2016 (2016)
Identification of anti-viral activity of the cardenolides, Na+/K+-ATPase inhibitors, against porcine transmissible gastroenteritis virus
Yang CW, et al.
Toxicology and Applied Pharmacology, 332, 129-137 (2017)
Buccal micronucleus cytome assay: results of an intra-and inter-laboratory scoring comparison
Bolognesi C, et al.
Mutagenesis, 30(4), 545-555 (2015)
Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes
Tunccer S, et al.
Scientific reports, 8(1), 14828-14828 (2018)
Single-molecule mRNA detection and counting in mammalian tissue
Lyubimova A, et al.
Nature Protocols, 8(9), 1743-1743 (2013)
Ver todos los artículos relacionados

Artículos

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Questions

1–9 of 9 Questions  

  1. DMSO is sensible to light? should I use a amber glass to storage my solution? Thank you.

    1 answer

    1. DMSO is sensitive to light and should be stored in amber or other dark colored containers or wrapped in foil to minimize exposure.

      Helpful?

  2. What is the DMSO dissolved in?

    1 answer

    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      Helpful?

  3. Where does DMSO come from?

    1 answer

    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  4. Is Dimethyl sulfoxide a sterile product?

    1 answer

    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  5. What is the difference between Dimethyl sulfoxide (DMSO) Products. D1435 and D2438?

    1 answer

    1. Products D1435 and D2438 both meet the USP specifications. The D2438 is sterile-filtered, but D1435 is not. Furthermore, the D2438 is quality control tested for endotoxin and use-tested for cell freezing.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer

    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

  8. What is the molarity of DMSO?

    1 answer

    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  9. Does DMSO have any synthetic utility?

    1 answer

    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

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