62287

Lipase A Candida antarctica, recombinant from Aspergillus oryzae

powder, beige, ~2 U/mg

• Número de CAS: 9001-62-1
• Comisión internacional de enzimas: 3.1.1.3 (BRENDA, IUBMB)
• Número CE: 232-619-9
• Número MDL: MFCD00131509
• Código UNSPSC: 12352204
• NACRES: NA.26

Propiedades

• recombinante: expressed in Aspergillus oryzae
• Nivel de calidad: 100
• Formulario: powder
• actividad específica: ~2 U/mg
• caducidad: limited shelf life, expiry date on the label
• color: beige
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Clave InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Descripción

Descripción general

Lipase A Candida antarctica (CALA) belongs to the α/β hydrolase family. The catalytic triad comprises Ser184, His366, and Asp334 residues.

Aplicación

Lipase A Candida antarctica, recombinant from Aspergillus oryzae has been used in the enzyme-catalyzed hydrolysis studies with 3-acetylthiohexanal. It has also been used as a biocatalyst to test its effect on the esterification of phytosterol with caprylic acid.

Acciones bioquímicas o fisiológicas

Lipase A Candida antarctica (CALA) is a thermostable serine hydrolase with a higher sn-2 preference towards triglycerides. It displays excellent activity towards large substrates as well as bulky sterically hindered tertiary alcohols.

Lipase A from Candida Antarctica catalyzes hydrolysis of tertiary alcohols to form glycerol and fatty acids and shows selectivity for the N-acylation of β-amino esters.

Definición de unidad

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314, as substrate); 1 U as described above is equivalent to ~0.15 U using trybutyrine, Cat. No. 91010, as substrate, at pH 8.0 and 70°C

Otras notas

Characterization

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)