227036

Cholera Toxin

from Vibrio cholerae, ≥95% (SDS-PAGE), solid, A-B type toxin, Calbiochem

• Sinónimos: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free
• Número de CAS: 9012-63-9
• Número MDL: MFCD00130776
• Código UNSPSC: 12352202
• NACRES: NA.51

Propiedades

• Nombre del producto: Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free, Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.
• Nivel de calidad: 100
• descripción: RTECS - LF3100000
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: do not freeze
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.

Useful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..

Useful for tissue culture applications.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
α-subunit of G-proteins

Product does not compete with ATP.

Reversible: no

Envase

Please refer to vial label for lot-specific concentration.

yes

Advertencia

Toxicity: Highly Toxic (H)

Forma física

Lyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.

Reconstitución

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.

Please refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.

Nota de análisis

Binding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.

single band by disc gel electrophoresis

Otras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Gilman, A.G. 1987. Annu. Rev.Biochem.56, 615.

Información legal

Not available for sale outside of the United States.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H300 + H310,H315
• Consejos de prudencia: P262 - P264 - P270 - P280 - P301 + P310 - P302 + P352 + P310
• Clasificaciones de peligro: Acute Tox. 1 Dermal - Acute Tox. 1 Oral - Skin Irrit. 2
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3