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N2752

Sigma-Aldrich

4-Nitrophenyl palmitate

lipase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

p-Nitrophenyl palmitate, Hexadecanoic acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C22H35NO4
CAS Number:
1492-30-4
Molecular Weight:
377.52
Beilstein:
1891754
EC Number:
216-084-9
MDL number:
MFCD00047732
UNSPSC Code:
12352204
PubChem Substance ID:
24897590
NACRES:
NA.83

product name

4-Nitrophenyl palmitate, lipase substrate

Assay

≥98% (TLC)

form

powder

solubility

chloroform: 100 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CCCCCCCCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3

InChI key

LVZSQWIWCANHPF-UHFFFAOYSA-N

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General description

4-Nitrophenyl palmitate is a substrate for lipase enzyme activity. Lipase hydrolyzes 4-nitrophenyl palmitate and yields the yellow colored product 4-nitrophenol, which is measurable spectrophotometrically at 410 nm. This method is advantageous due to its short reaction time and facile spectrophotometric analyses. The cell-bound lipase has preference for 4-nitrophenyl palmitate as substrate than the extracellular lipase.

Application

4-nitrophenyl palmitate has been used as a substrate for lipase enzyme activity.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Precautionary Statements

P280

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M M Maia et al.
Bioresource technology, 76(1), 23-27 (2001-04-24)
Lipase (Glycerol ester hydrolase EC 3.1.1.3.) from a Brazilian strain of Fusarium solani FSI has been investigated. The effect of different carbon sources and trace elements added to basal medium was observed with the aim of improving enzyme production. Lipase
Shamoon Asmat et al.
Materials science & engineering. C, Materials for biological applications, 99, 25-36 (2019-03-21)
Herein, as a promising support, a magnetic enzyme nanoformulation have been designed and fabricated by a poly-o-toluidine modification approach. Owing to the magnetic nature and the existence of amine functionalized groups, the as-synthesised poly(o-toluidine) functionalized magnetic nanocomposite (Fe3O4@POT) was employed
Lipase and biosurfactant from Ochrobactrum intermedium strain MZV101 isolated by washing powder for detergent application
Zarinviarsagh M, et al.
Lipids in Health and Disease, 16(1), 177-177 (2017)
Differences in hydrolytic abilities of two crude lipases from Geotrichum candidum 4013
Brabcova J, et al.
Yeast, 27(12), 1029-1038 (2010)
Microbial enzymatic activities in aerobic activated sludge model reactors
Li Y and Chrost RJ
Enzyme and Microbial Technology, 39(4), 568-572 (2006)
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