Skip to Content
Merck
All Photos(1)

Key Documents

M217

Sigma-Aldrich

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α1, α3, α5 Subunits) antibody produced in rat

clone mAb 35, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-ACHR

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

mAb 35, monoclonal

form

buffered aqueous solution

species reactivity

human, chicken, eel

should not react with

Xenopus

technique(s)

immunohistochemistry (frozen sections): 1:3,000

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Specificity

Binds to the main immunogenic region on the extracellular surface of the α1 subunit of muscle AChR′s and to homologous regions on α3 and α5 subunits of neuronal AChR′s. The epitope is located on the extracellular surface of the α1 subunit of the muscle nicotinic acetylcholine receptor. The antibody binds to denatured chick α5 AChR, but does not bind well to denatured α3, α4, α7, α8, and β2. It may also bind β3 due to homology of main immunogenic region. May be used to localize and detect the α1, α3 and α5 subunits of the nicotinic acetylcholine receptor (AChR).

Immunogen

nicotinic acetylcholine receptor from the electric eel Electrophorus.

Physical form

Solution in 20 mM Na3PO4, pH 7.2, containing 150 mM NaCl and 0.05 % sodium azide.

Legal Information

Sold with permission of the Salk Institute

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jee-Young Park et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(31), 10211-10218 (2014-08-01)
Mutations in AChR subunits, expressed as pentamers in neuromuscular junctions (NMJs), cause various types of congenital myasthenic syndromes. In AChR pentamers, the adult ε subunit gradually replaces the embryonic γ subunit as the animal develops. Because of this switch in

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service