Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutated during growth in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.
Maintain cultures between 4-5x100,000 cells/ml; 5% CO2; 37°C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.
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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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