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Key Documents

53B3

Application: Fusion partner, 85062501

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Bovine Heterohybridoma

ImmunoDon

Jersey calf lymph node

Myeloma

53B3 (mouse x bovine heteromyeloma)

Reactivity

Application: Fusion partner

technique(s)

cell culture | mammalian: suitable

isotype

Not specified

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse x Bovine 53B3 x Jersey calf lymph node

Cell Line Description

This HAT sensitive line can be used as a fusion partner in the production of heterohybridomas.

Immunogen

Sheep red blood cells

Application

Fusion partner

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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