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86250

Sigma-Aldrich

α-Amylase from Aspergillus oryzae

greener alternative

powder, ~1.5 U/mg (~0.2 U acc. to Willstätter)

Synonym(s):

Taka-Amylase A, Taka-Diastase from Aspergillus oryzae, 1,4-α-D-Glucan-glucanohydrolase

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About This Item

Enzyme Commission number:
3.2.1.1 (BRENDA, IUBMB)
EC Number:
232-588-1
MDL number:
MFCD00081319
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Aspergillus sp. (A. oryzae)

Quality Level

100

form

powder

specific activity

~1.5 U/mg (~0.2 U acc. to Willstätter)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Enabling,

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25 °C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Molecular weight; Product distribution in the hydrolysis of amylose; Application in (selective) hydrolysis/condensation of glycosidic bonds; Suitable for use in thiamine determination.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H334

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T Takagi
Journal of biochemistry, 89(2), 363-368 (1981-02-01)
Molecular weight of Aspergillus oryzae alpha-amylase (Taka-amylase A) was estimated to be 51,000 +/- 500 by the combined use of high pressure silica gel (TSK-GEL G3000SW) chromatography and the low angle laser light scattering technique. The study was carried out
Mingtao Huang et al.
Nature communications, 8(1), 1131-1131 (2017-10-27)
The biotech industry relies on cell factories for production of pharmaceutical proteins, of which several are among the top-selling medicines. There is, therefore, considerable interest in improving the efficiency of protein production by cell factories. Protein secretion involves numerous intracellular
Product distribution in amylase-catalyzed hydrolysis of amylose. Comparison of experimental results with theoretical predictions.
H Kondo et al.
Journal of biochemistry, 87(4), 1053-1070 (1980-04-01)
K. Fujita et al.
Bulletin of the Chemical Society of Japan, 62, 3150-3150 (1989)
P W Defibaugh
Journal - Association of Official Analytical Chemists, 70(3), 514-517 (1987-05-01)
Seven commercially available enzymes were studied for suitability as substitutes in the AOAC thiamine determination, because the enzyme Takadiastase used in the method has not been available since 1976, and alternative enzymes were likewise unavailable or unsuitable for releasing thiamine
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