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Phosphatase, Acid from potato

Name: Phosphatase, Acid from potato
Brand: SIGMA

lyophilized powder, ≥0.5 unit/mg solid

Synonym(s):

(Orthophosphoric-monoester phosphohydrolase acid optimum), Acid Phosphatase from potato

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About This Item

CAS Number:

9001-77-8

eCl@ss:

42010102

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-630-9

MDL number:

MFCD00131847

EC Number:

3.1.3.2 (BRENDA, IUBMB)

Specific activity:

≥0.5 unit/mg solid

Biological source:

potato

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Properties

biological source

potato

Quality Segment

200

form

lyophilized powder

specific activity

≥0.5 unit/mg solid

mol wt

69 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

SMILES string

O(CC(O)C)CC#C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI key

GZCWLCBFPRFLKL-UHFFFAOYSA-N

Description

Application

Acid phosphatase is routinely used to dephosphorylate proteins and nucleic acids. Acid phosphatase is an acid hydrolase that normally resides in lysosomes. It is used as marker for the identification of lysosomes in subcellular fractionations. Acid phosphatase, product P3752, has been used as a control in order to study how Mycobacterium bovis BCG effects the levels of acid phosphatase activity in alveolar macrophages.

Biochem/physiol Actions

Acid phosphatase is a cytoplasmic lysosomal hydrolase that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 4.8 at 37 °C.

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This Item	SRE0048	P1146	524529
Sigma-Aldrich P3752 Phosphatase, Acid from potato	Sigma-Aldrich SRE0048 Phosphatase, Acid from potato	Sigma-Aldrich P1146 Phosphatase, Acid from potato	Sigma-Aldrich 524529 Phosphatase, Acid, Potato
description lyophilized powder, ≥0.5 unit/mg solid	description Suitable for manufacturing of diagnostic kits and reagents	description lyophilized powder, ≥3.0 units/mg solid	description -
biological source potato	biological source -	biological source -	biological source -
specific activity ≥0.5 unit/mg solid	specific activity 3.0-10.0 unit/mg solid	specific activity ≥3.0 units/mg solid	specific activity ≥2 U/mg
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized
application(s) diagnostic assay manufacturing	application(s) -	application(s) -	application(s) -
mol wt 69 kDa	mol wt -	mol wt 69 kDa	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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