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T1949

Sigma-Aldrich

Total Protein Reagent

Synonym(s):

Biuret Reagent

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500 ML
€132.00

€132.00


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500 ML
€132.00

About This Item

UNSPSC Code:
12352202
NACRES:
NC.07

€132.00


Please contact Customer Service for Availability

Request a Bulk Order

Quality Level

Application

Total Protein Reagent can be used for the quantitative, colorimetric determination of total protein concentration in solution at 540 nm. The total protein reagent is supplied as a ready-to-use liquid.
Total Protein Reagent has been used in the biuret assay.[1][2]

Biochem/physiol Actions

A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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B T Doumas et al.
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We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau
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The Journal of Biological Chemistry, 177, 759-759 (1949)
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