SRE0007

Mutanolysin from Streptomyces globisporus ATCC 21553

Suitable for manufacturing of diagnostic kits and reagents, ≥4000 units/mg protein (biuret)

• Synonym(s): Mutanolysin
• CAS Number: 55466-22-3
• MDL number: MFCD00131635
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Streptomyces globisporus)
• Quality Level: 400
• form: lyophilized powder
• specific activity: ≥4000 units/mg protein (biuret)
• mol wt: 23 kDa
• concentration: ≥40% protein
• suitability: suitable for cell lysis
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. It lyses Listeria and other gram positive bacteria such as Lactobacillus and Lactococcus.

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Unit Definition

One unit will produce a ΔA₆₀₀ of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll^® and sodium succinate buffer salts

Legal Information

Ficoll is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1