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Key Documents

P3250

Sigma-Aldrich

Papain from papaya latex

lyophilized powder, 0.5-2.0 units/mg solid

Synonym(s):

Papainase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42030311
Pricing and availability is not currently available.

form

lyophilized powder

specific activity

0.5-2.0 units/mg solid

mol wt

23.4 kDa

storage temp.

2-8°C

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Application

Papain papaya latex, product P3250, has been used to hydrolyze chitosan to determine whether chitosan can be used in food preservation[1]. It is used in dissecting solutions[2]. It is used to produce Fab fragments of antibodies.[3] It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.[4][5]
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans[1]. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides.

Unit Definition

One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C

Other Notes

View more information on papain at www.sigma-aldrich.com/enzymeexplorer.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Zonghui Dai
Contrast media & molecular imaging, 2022, 2869707-2869707 (2022-06-11)
The aim of the study was to study the protective effect of the Rhizoma Drynariae-Epimedium formula on osteoarthritis in rats and to explore its mechanism. Fifty SD rats were randomly divided into 5 groups, namely, the control group, model group
H Guo et al.
The Journal of physiology, 537(Pt 1), 115-123 (2001-11-17)
1. It has been proposed that the activation of NMDA receptors and upregulation of protein kinase C (PKC) underlie the exaggerated and persistent pain experienced in the inflammatory state. However, there is no direct evidence to show that inflammation alters
J Rhoades et al.
Applied and environmental microbiology, 66(1), 80-86 (2000-01-05)
The objective of this study was to determine whether chitosan (poly-beta-1,4-glucosamine) and hydrolysates of chitosan can be used as novel preservatives in foods. Chitosan was hydrolyzed by using oxidative-reductive degradation, crude papaya latex, and lysozyme. Mild hydrolysis of chitosan resulted
H Cai et al.
Analytical chemistry, 70(3), 580-589 (1998-02-01)
The third member of a new family of single-isomer charged cyclodextrins, the sodium salt of heptakis(2,3-dimethyl-6-sulfato)-β-cycldextrin, has been synthesized, characterized, and used for the capillary electrophoretic separation of the enantiomers of neutral, acidic, basic, and zwitterionic analytes. Though heptakis(2,3-dimethyl-6-sulfato)-β-cyclodextrin complexes
Yonghua Bi et al.
Journal of vascular and interventional radiology : JVIR, 23(11), 1529-1536 (2012-10-30)
The objective of this study was to determine the possibility of creating a novel animal model of abdominal aortic aneurysm (AAA) in rabbits by the periarterial application of papain. Twelve New Zealand white rabbits were randomized into two groups: (1)

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