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Key Documents

N7271

Sigma-Aldrich

α(2→3) Neuraminidase from Streptococcus pneumoniae

buffered aqueous solution

Synonym(s):

Acyl-neuraminyl Hydrolase, Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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.2 UNIT
€1,070.00

€1,070.00


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.2 UNIT
€1,070.00

About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

€1,070.00


Please contact Customer Service for Availability

biological source

Streptococcus pneumoniae

Quality Level

form

buffered aqueous solution

concentration

≥5 units/mL

foreign activity

β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected

shipped in

wet ice

storage temp.

2-8°C

Gene Information

Streptococcus pneumoniae R6 ... nanB(933504)

Biochem/physiol Actions

Releases α(2→3)-linked N-acetylneuraminic acid from complex oligosaccharides.

Packaging

Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).

Unit Definition

One unit will hydrolyze 1μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Preparation Note

Expressed in glycosidase-free hosts.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Chinh Tran-To Su et al.
BMC bioinformatics, 14 Suppl 16, S7-S7 (2014-02-26)
Since late March 2013, there has been another global health concern with a sudden wave of flu infections by a novel strain of avian influenza A (H7N9) virus in China. To-date, there have been more than 100 infections with 23
Tadatsugu Imamura et al.
Journal of virology, 88(5), 2374-2384 (2013-12-29)
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying
Alana L Woodward et al.
Veterinary microbiology, 169(3-4), 113-127 (2014-02-01)
Equine influenza viruses are a major cause of respiratory disease in horses worldwide and undergo antigenic drift. Several outbreaks of equine influenza occurred worldwide during 2010-2012, including in vaccinated animals, highlighting the importance of surveillance and virus characterisation. Virus isolates
S Bhatt et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1614), 20120382-20120382 (2013-02-06)
Few questions on infectious disease are more important than understanding how and why avian influenza A viruses successfully emerge in mammalian populations, yet little is known about the rate and nature of the virus' genetic adaptation in new hosts. Here

Articles

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

Questions

1–5 of 5 Questions  
  1. What is the long term storage and shipping condition of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. It is shipped at ambient temperature, but it is to be stored at 2-8 °C for long term storage. Do not FREEZE as it is in a buffered aqueous solution.

      Helpful?

  2. What is the molecular weight of ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. The molecular weight of this ?(2—›3 Neuraminidase enzyme is approximately 75 kDa.

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  3. What are the reaction conditions of the enzyme ?(2—›3) Neuraminidase, Product No. N7271?

    1 answer
    1. One nanomole of substrate or less can be cleaved in a 20 μL reaction volume. In the standard reaction use: 4 μL of 5X enzyme reaction "buffer B" (=250 mM sodium phosphate buffer, pH 6.0), a combined volume of 14 μL containing purified oligosaccharide and water and 2 μL of NANase I (N7271). Incubate 1 hour at 37 °C. To cleave more than one nanomole of substrate, increase reaction volume and enzyme proportionally.

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  4. For ?(2—›3) Neuraminidase, Product No. N7271, what is the optimum pH range for the neuraminidase enzyme activity?

    1 answer
    1. The optimum pH range for ?(2—›3 neuraminidase is 6.0. Please see the data sheet.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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