The Mayers Hematoxylin available is the MHS series of hematoxylin solutions, comeS in various sizes from 100ml to 4L. These solutions are all formulated with chloral hydrate and no alcohol. There is also a Mayers Hemalum solution available as product number 109249, which is formulated with ethylene glycol in place of chloral hydrate. Additionally, there is an Acid Hematoxylin formulated with chloral hydrate, with catalog number 2852-100ml.
MHS16
Mayer′s Hematoxylin Solution
Synonym(s):
(6aS,11bR)-7,11b-Dihydroindeno[2,1-c]chromene-3,4,6a,9,10(6H)-pentol, Delafield′s hematoxylin, Progressive stains
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About This Item
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form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
1 g/L
technique(s)
microbe id | staining: suitable
pH
2.4 (25 °C)
application(s)
hematology
histology
storage temp.
room temp
SMILES string
Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O
InChI
1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1
InChI key
WZUVPPKBWHMQCE-XJKSGUPXSA-N
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General description
Used as a counterstain for procedures such as immunohistochemistry or laser microdissection.
Application
General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.
Other Notes
1 g/L certified hematoxylin
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Related Content
Learn about the criticality of biological tissue staining for research and clinical pathology using standard and special stains and dyes.
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Is there an alternative product available for the MHS series of Mayers Hematoxylin solutions?
1 answer-
Helpful?
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What would be the best solution to dilute product MHS16 to a 50% concentration?
1 answer-
Aluminum-based hematoxylin solutions, including Mayers Hematoxylin, should never be diluted before use. Mayers Hematoxylin solution is ready to use as it comes from the bottle. The issue with diluting aluminum-based hematoxylins is that while the hematoxylin strength is diluted, so is the aluminum concentration. Mayers hematoxylin is a progressive hematoxylin, formulated with enough aluminum to prevent the hematoxylin from nonspecifically binding with the cytoplasm. Diluting the aluminum may lead to staining of the cytoplasm by the hematoxylin. As Mayers Hematoxylin has one of the lower hematoxylin concentrations among popular hematoxylin formulations, the best solution is to simply reduce the amount of time the tissues are stained in the Mayers Hematoxylin.
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What is the recommended bluing agent for MHS16? Also, what is the recommended range of incubation time for using MHS16?
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The protocol for use simply states to blue in running tap water or a dilute alkaline solution. Warm tap water will be slightly more alkaline than cold tap water. Opt to use dilute alkaline solutions if the pH of tap water tends towards the acidic side, as this could cause issues with the tissues bluing quickly and properly. Among these solutions are saturated lithium carbonate solutions or 15 grams per liter of sodium bicarbonate, which can produce a slightly alkaline solution. Another option is Scott's Tap Water Substitute (catalog number S5134). Reagent or ACS grade lithium carbonate and sodium bicarbonate are suitable for use. Bluing with Scott's Tap Water Substitute produced the most stable stained nuclei. As for the recommended staining time, it is 15 minutes. However, Mayers Hematoxylin is a progressive hematoxylin, so if the nuclear staining is not intense enough, the staining time can be increased. Extending the staining time past 15 minutes should not result in overstaining of the cytoplasm, as Mayers Hematoxylin is formulated to prevent this.
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There are several Mayer's Hematoxylins listed on the website, beginning with the letters MHS. What are the differences?
1 answer-
Products MHS1, MHS16, MHS32, MHS80 and MHS128 are in fact the same product. In this case a unique product number was assigned to each package size. Other than the package size, there are no differences in formulation or use.
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Is chloral hydrate used in the preparation of Mayer's Hematoxylin? Does this mean the solution is controlled as a DEA Schedule IV product?
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Yes, chloral hydrate is included in the formulation. No, the solution is not controlled as a DEA Schedule IV product. Once the choral hydrate is mixed with the remaining chemicals, it is impossible to obtain the chloral hydrate in a purified form.
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The website states that Mayer's Hematoxylin solution is a progressive type hematoxylin. What is a progressive type hematoxylin?
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Hematoxylin solutions are generally classified as either progressive or regressive. Progressive formulations will stain only the nucleus. No staining should occur within the cytoplasm. Regressive hematoxylins have higher dye contents and if used for sufficient periods of time will overstain both the nucleus and the cytoplasm. Acid differentiation is required to remove the excessive stain from the cytoplasm and nucleus. This differentiation take practice to master. Too much or too little stain can be removed during differentiation, resulting in poorly stained slides. For users lacking extensive experience with the Hematoxylin and Eosin staining procedure, progressive hematoxylin formulations are often the better choice.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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There is also a product listed on the website as Hematoxylin Solution According to Mayer. What is the difference between product 51275 and the various Mayer's Hematoxylin solutions?
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The basic formulations are the same. Both include chloral hydrate, although some of the concentrations could vary slightly. Product 51275 is not intended for In Vitro Diagnostic Use. Product 51275 is not CE Marked. The various Mayer's Hematoyxlin solutions are intended For In Vitro Diagnostic Use and are also CE Marked. Product inserts are available for various languages.
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The slides were stained with Mayer's Hematoxylin but the nuclei are not blue. Why?
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The nuclei will originally appear as the same color as the solution. Only after the slides have been "blued" will the nuclei appear blue. The pH of the solution is acidic. This acidic pH promotes specific staining. However the slides must be washed in an neutral or alkaline solution for the color to shift to blue. Prolonged warm water washes in tap water are often sufficient for the bluing to occur. To shorten the time spent in washing, various bluing agents are often used to shorten the blueing process. Scott's Tap Water Substitute (Product No. S5134) is one such blueing agent.
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