J4500

Anti-c-Jun N-Terminal Kinase antibody produced in rabbit

whole antiserum

• Synonym(s): Anti-JNK1, JNK2
• MDL number: MFCD00676444
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: antigen, JNK1 46 kDa; antigen, JNK2 55 kDa
• contains: 15 mM sodium azide
• species reactivity: rat, human, mouse
• technique(s): microarray: suitable; western blot: 1:16,000 using rat brain extract and mouse NIH-3T3 fibroblast extract, respectively; western blot: 1:2,000 using mouse NIH 3T3 fibroblast cell lysate
• UniProt accession no.: P45983
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPK8(5599) ; mouse ... Mapk8(26419) ; rat ... Mapk8(116554)

Description

General description

Anti-c-Jun N-Terminal Kinase (JNK1, JNK2) is developed in rabbit using a synthetic peptide, corresponding to amino acids of human c-Jun N-terminal kinase 1 (JNK1), coupled to KLH as the immunogen.

JNK (c-Jun N terminal protein kinase, also known as stress activated protein kinase, SAPK1) is a protein that belongs to serine/threonine-protein kinase family. It has a critical role in inhibiting WOX1 (WW domain-containing oxidoreductase) mediated apoptosis. It also mediates starvation-induced BCL2 phosphorylation and activates autophagy. Anti-c-Jun N-terminal kinase antibody can be used in microarray. Rabbit anti-c-Jun N-terminal kinase antibody reacts specifically with JNK1 (46 kD) and JNK2 (55 kD) of cell culture lysates and rat brain tissue extracts. The product has also shown weak cross reactivity for JNK2β (50 kD) isoform in mouse NIH-3T3 fibroblast cell lysate.

Immunogen

Synthetic peptide corresponding to amino acids 339-354 of human JNK1 (c-Jun N-terminal kinase 1), conjugated to KLH. The sequence is highly conserved in JNK1, JNK2 α, β, γ (p54 SAPK α, β, γ) and identical in human, rat, and mouse JNK1.

Application

Anti-c-Jun N-Terminal Kinase antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases which play a central role in mitogenic signaling. The MAPKs function to transduce extracellular signals to intracellular targets, including transcription factors controlling the expression of genes essential to many cellular processes including proliferation, development and differentiation. JNK1 is essential for tumor necrosis factor alpha (TNF-α)-induced c-Jun kinase activation, c-Jun expression, and apoptosis. JNK1 and JNK2 participates in the survival of neuronal cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable