A search of the website does not show a density for product G4155. For products supplied in small quantities, it is not common for the density to be measured. However, for solutions available in larger quantities, density information is often provided. Although the density for G4155 is not stated, there is some useful information available from the website. The physical form of the product is a suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, and 0.5 mM mercaptoethanol at pH 7.4.
G4155
β-Galactosidase from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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1000 UNITS
€109.00
3000 UNITS
€280.00
5000 UNITS
€399.00
€109.00
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1000 UNITS
€109.00
3000 UNITS
€280.00
5000 UNITS
€399.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
€109.00
Please contact Customer Service for Availability
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form
aqueous glycerol suspension
Quality Level
specific activity
≥500 units/mg protein (biuret)
mol wt
465 kDa
storage temp.
−20°C
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Related Categories
Application
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Physical properties
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Unit Definition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Physical form
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Other Notes
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Could you please advise if the density of the aqueous suspension of beta galactosidase from Escherichia coli (G4155) can be assumed as 1 uL of solution is equal to 1 mg, given that the SDS and provided enzyme assay do not contain this information?
1 answer-
Helpful?
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We recently purchased this enzyme. Information given regarding enzyme volume is only 1000 units. I want to know what would be units per uL. How much is the liquid volume in the given product to calculate the enzyme concentration? Provide proper informatio
1 answer-
Units/ml can be calculated using the numbers reported in batch specific CofA. Below is an example.
CofA of G4155, lot 0000303635
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G4155-3KU0000303635.pdf
1.5 mgP/ml X 766 units/mg Protein = 1149 units/mlHelpful?
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