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EMS0005

Sigma-Aldrich

SOLu-Trypsin Dimethylated

Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution

Synonym(s):

Dimethylated Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Dimethylated rTrypsin, Mass Spectrometry Trypsin Dimethlyated, No chemotryptic activity

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100 μG
€148.00
4 X 100 μG
€401.00

€148.00

Please contact Customer Service for Availability
Request a Bulk Order
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100 μG
€148.00
4 X 100 μG
€401.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.78

€148.00

Please contact Customer Service for Availability
Request a Bulk Order

recombinant

expressed in Pichia pastoris

Quality Level

200

grade

Proteomics Grade

form

ready-to-use solution

potency

>10,000 units/mg

specific activity

≥10,000 units/mg protein (Enzymatic activity)

suitability

suitable for mass spectrometry

UniProt accession no.

P00761

shipped in

wet ice

storage temp.

2-8°C

General description

SOLu-Trypsin Dimethylated (EMS0005) is our exclusive, Advanced Proteomics Grade enzyme that is solution stable for mass spectrometry. Designed to be stable in solution when refrigerated, SOLu-Trypsin Dimethylated can be used immediately without preparation. Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately. SOLu-Trypsin Dimethylated allows excess product to be saved for future use, thus eliminating unnecessary waste and cost. SOLu-Trypsin Dimethylated is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

Features and Benefits

  • Ready to use - no preparation, such as reconstitution or thawing, is required
  • Fits seamlessly into established workflow - no need to modify protocols
  • Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
  • Recombinant, porcine sequence - no chymotryptic activity
  • Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000404852
0000404852
SLCP2792
SLCL5387
SLCK9305

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Articles

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

Questions

  1. What's the feature/benefit of EMS0005 over EMS0004? Thanks!

    1 answer

    1. The difference between SOLu-Trypsin Dimethylated (EMS0005) and SOLu-Trypsin (EMS0004) products is primarily the stability. The dimethylated form, EMS0005, is more resistant to autolysis over time.

      Please see the links below to review the datasheet for each product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/535/ems0005bul.pdf
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/334/377/ems0004bul.pdf

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