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A8011

Sigma-Aldrich

Antifoam C Emulsion

aqueous-silicone emulsion

Synonym(s):

Antifoaming Agent Emulsion

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About This Item

UNSPSC Code:
12161900
NACRES:
NA.25
Pricing and availability is not currently available.

biological source

synthetic

Quality Level

200

form

emulsion

contains

emulsifier

technique(s)

cell culture | hybridoma: suitable
microbiological culture: suitable

mp

0  °C (32 °F)

Related Categories

General description

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion[1] consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.[1]

Application

Antifoam C emulsion has been used:
  • as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture[2]
  • as a component of feed medium[2],
  • as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations[3]
  • as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions[4]

Features and Benefits

  • Minimizes compatibility issues with biological systems
  • Highly effective defoamer at low concentrations
  • Highly effective and versatile silicone defoamer

Other Notes

A 30% aqueous emulsion of polydimethylsiloxane concentrate.
For additional information on our range of Biochemicals, please complete this form.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319,H335

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000388813
0000388814
0000384373
0000384371
0000302044

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Magdalena Pappenreiter et al.
Frontiers in bioengineering and biotechnology, 7, 195-195 (2019-09-10)
In aerobic cell cultivation processes, dissolved oxygen is a key process parameter, and an optimal oxygen supply has to be ensured for proper process performance. To achieve optimal growth and/or product formation, the rate of oxygen transfer has to be
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
Characterizing the Effects of Antifoam C Emulsion on Oxygen Mass Transfer within the BIOne Drilled-Hole Sparger and Microsparger Single-Use Bioreactor Systems
McAndrew J and Kauffman G
American Pharmacy null
Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
Gang Fu et al.
Biotechnology and applied biochemistry, 67(5), 812-818 (2019-10-08)
d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Will there be a problem if I add 100ppm of antifoam when cultivating bacteria?

    1 answer

    1. This product is typically effective at 1-10 ppm. Therefore, there is no guarantee how 100 ppm will perform for bacteria cultivation.

      Helpful?

  4. Can the antifoam be autoclaved pure? When diluted (e.g. to 0.5% or 0.1%)?

    1 answer

    1. Yes, this product may be autoclaved. Autoclaving the pure product may result in phase separation and might require remixing the emulsion.

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