This product is a 30% solution in water. The active substances are chemically identified as C12-14-Alkyldimethyl-betaines. Please see the link below to review the product Safety Data Sheet:
https://www.sigmaaldrich.com/sds/sigma/30326
30326
EMPIGEN® BB detergent
~30% active substance
Synonym(s):
N,N-Dimethyl-N-dodecylglycine betaine, N-(Alkyl C10-C16)-N,N-dimethylglycine betaine
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50 ML
€51.90
250 ML
€123.00
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50 ML
€51.90
250 ML
€123.00
About This Item
Linear Formula:
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
CAS Number:
Beilstein:
3670807
EC Number:
MDL number:
UNSPSC Code:
12161900
PubChem Substance ID:
NACRES:
NA.25
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description
zwitterionic
Quality Level
form
liquid
composition
solid content, 35.0-45.0 % (w/v)
concentration
~30% active substance
CMC
1.6-2.1 mM (20-25°C)
density
1.030-1.060 g/mL at 20 °C
UV absorption
λ: 417 nm Amax: ≤0.055
λ: 441 nm Amax: ≤0.037
SMILES string
CCCCCCCCCCCC[N+](C)(C)CC([O-])=O
InChI
1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3
InChI key
DVEKCXOJTLDBFE-UHFFFAOYSA-N
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Application
Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.
Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity[1]. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease[2]. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses[3].
Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity[1]. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease[2]. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses[3].
Legal Information
EMPIGEN is a registered trademark of Huntsman Corporation
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Y Rinke et al.
Virology, 170(2), 424-432 (1989-06-01)
We have established a sensitive assay for the quantitative determination of large T antigen determinants on the surface of living simian virus 40 (SV40)-transformed cells (mKSA). Cells in suspension culture were incubated with monoclonal antibodies specific for large T antigen
James K Wahl
Hybridoma and hybridomics, 21(1), 37-44 (2002-05-07)
Desmosomes are the most prominent cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Desmogleins and desmocollins are the transmembrane core of the desmosome and both are members of the cadherin family
Islam Khan et al.
Microbes and infection, 7(5-6), 853-859 (2005-05-10)
Campylobacter jejuni is a major food-borne pathogen and a leading cause of diarrhoea. A cytotoxin is most likely involved in the pathogenesis of inflammatory diarrhoea due to C. jejuni. A 45-kDa outer membrane protein encoded by the porA gene was
Wenjin Guo et al.
Protein expression and purification, 90(1), 34-39 (2013-05-15)
Vaccinia virus (VV) has been used to express a variety of heterologous proteins, including HIV envelope (Env) glycoproteins. The Env protein is synthesized as a precursor molecule, gp160, which is cleaved into the surface antigen gp120 and the transmembrane protein
R Jennings et al.
Journal of medical virology, 31(2), 98-108 (1990-06-01)
HSV-1 antigen preparations solubilised from Vero cells by using either the non-ionic detergent Nonidet P40 or the zwitterionic detergent Empigen BB, and purified on sucrose density gradients or over a sucrose cushion, were tested by ELISA with anti-HSV-1 glycoprotein monoclonal
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