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04529

Sigma-Aldrich

Nitrilase

recombinant, expressed in E. coli, ≥2.0 U/mg

Synonym(s):

Nitrile aminohydrolase

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10 MG
€92.80
50 MG
€357.00

€92.80

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10 MG
€92.80
50 MG
€357.00

About This Item

CAS Number:
9024-90-2
Enzyme Commission number:
3.5.5.1 (BRENDA, IUBMB)
MDL number:
MFCD04040969
UNSPSC Code:
12352204
NACRES:
NA.54

€92.80

Please contact Customer Service for Availability

recombinant

expressed in E. coli

Quality Level

100

form

crystals
powder

specific activity

≥2.0 U/mg

mol wt

41 kDa

storage temp.

2-8°C

General description

Nitrilase belongs to the C-N hydrolase superfamily.[1] It possesses the catalytic triad motifs: glutamate, lysine, and cysteine and are distributed in fungi, plants, and animals.[2]The isoelectric point (pI) of this protein is 8.1. Native polyacrylamide gel electrophoresis (PAGE).

Application

Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.[3]

Biochem/physiol Actions

Nitrilases catalyze the liberation of ammonia and the formation of carboxylic acid from the nitriles.[4] The oligomeric structural organization of nitrilase is crucial for its functionality.[1] It displays broad substrate specificity.[1]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK7616
BCCG3189
BCCD3763
BCCD2216
BCCC3420

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Y V D Nageshwar et al.
Bioprocess and biosystems engineering, 34(5), 515-523 (2010-12-29)
Microbial nitrilases are biocatalysts of interest and the enzyme produced using various inducers exhibits altered substrate specificity, which is of great interest in bioprocess development. The aim of the present study is to investigate the nitrilase-producing Alcaligenes faecalis MTCC 10757
Zhi-Qiang Liu et al.
PloS one, 8(6), e67197-e67197 (2013-07-05)
In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase
Zhi-Jun Zhang et al.
Journal of biotechnology, 152(1-2), 24-29 (2011-01-26)
The enantioselective hydrolysis of mandelonitrile with whole cells of a recombinant Escherichia coli expressing nitrilase activity was severely inhibited by the substrate at high concentrations (>300mM), which resulted in a low yield of the target product (R)-(-)-mandelic acid. To relieve
Chunsheng Yang et al.
Applied biochemistry and biotechnology, 165(7-8), 1556-1567 (2011-09-23)
The characteristics of the new nitrilase-producing strain Rhodobacter sphaeroides LHS-305 were investigated. By investigating several parameters influencing nitrilase production, the specific cell activity was ultimately increased from 24.5 to 75.0 μmol g(-1) min(-1), and hereinto, the choice of inducer proved
Pablo Soriano-Maldonado et al.
Applied and environmental microbiology, 77(16), 5761-5769 (2011-06-28)
Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate
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Questions

1–3 of 3 Questions  

  1. what is the bacteria or organism whose genome sequence have been expressed in E.Coli for nitrilase procurement? I need the name of the organism for research purpose

    1 answer

    1. This enzyme is a recombinant protein produced in e. coli. The biological source and sequence of the protein is considered proprietary and may not be shared.

      Helpful?

  2. Can we get the source of the bacteria or organism whose genome sequence is being used for recombinant expressing in E.Coli for procuring nitrilase. Used for research purspose by a student

    1 answer

    1. Unfortunately, the source and sequence is considered proprietary.

      Helpful?

  3. Is the nitrilase been synthesized through DNA recombinant technology or is there any other source organism from which the nitrilase has been procured?

    1 answer

    1. Yes, this product is a recombinant protein expressed in E. coli.

      Helpful?

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