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D1435

Sigma-Aldrich

Dimethyl sulfoxide

meets EP testing specifications, meets USP testing specifications

Synonym(s):

DMSO

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500 ML
€356.00
1 L
€596.00
4 L
€1,460.00

€356.00


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500 ML
€356.00
1 L
€596.00
4 L
€1,460.00

About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.71

€356.00


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Agency

meets EP testing specifications
meets USP testing specifications

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

Assay

≥99.5%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cryopreservation: suitable

impurities

≤0.1% water (Karl Fischer, USP)
≤0.2% water (Karl Fischer, EP)

color

colorless

refractive index

n20/D 1.478-1.479
n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.100-1.104 g/mL at 25 °C (relative density (EP))
1.10 g/mL (lit.)

UV absorption

λ: 285 nm Amax: ≤0.20
λ: 295 nm Amax: ≤0.20

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Application

Dimethyl sulfoxide has been used:
  • for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments[1]
  • as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells[2]
  • for the suspension and separation of buccal cells[3]
  • a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Biochem/physiol Actions

Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant.[4] It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.[5]

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes
Tunccer S, et al.
Scientific reports, 8(1), 14828-14828 (2018)
Single-molecule mRNA detection and counting in mammalian tissue
Lyubimova A, et al.
Nature Protocols, 8(9), 1743-1743 (2013)
Buccal micronucleus cytome assay: results of an intra-and inter-laboratory scoring comparison
Bolognesi C, et al.
Mutagenesis, 30(4), 545-555 (2015)
Comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood
Chen G, et al.
Stem Cells International, 2016 (2016)
Identification of anti-viral activity of the cardenolides, Na+/K+-ATPase inhibitors, against porcine transmissible gastroenteritis virus
Yang CW, et al.
Toxicology and Applied Pharmacology, 332, 129-137 (2017)

Articles

Overview of good cell banking practices for cell line cryopreservation purposes.

Questions

1–9 of 9 Questions  
  1. DMSO is sensible to light? should I use a amber glass to storage my solution? Thank you.

    1 answer
    1. DMSO is sensitive to light and should be stored in amber or other dark colored containers or wrapped in foil to minimize exposure.

      Helpful?

  2. What is the difference between Dimethyl sulfoxide (DMSO) Products. D1435 and D2438?

    1 answer
    1. Products D1435 and D2438 both meet the USP specifications. The D2438 is sterile-filtered, but D1435 is not. Furthermore, the D2438 is quality control tested for endotoxin and use-tested for cell freezing.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  5. Is Dimethyl sulfoxide a sterile product?

    1 answer
    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

  6. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  7. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  8. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

  9. What is the DMSO dissolved in?

    1 answer
    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

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