Safranin O (C.I. 50240) for microscopy Certistain®, is a dry dye that is used for the preparation of a staining solution for e.g. Gram stain procedures in smears and sections of human origin in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Gram-negative bacteria will be counterstained by Gram′s safranin O solution and appear orange. As a CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.
Application
for microscopy Certistain®
Analysis Note
Dye content (spectrophotometrically): ≥ 80 % Identity (UV/VIS-Spectrum): passes test Absorption maximum λ max (ethanol 50 %): 530 - 534 nm Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1260 - 1570 TLC-Test: passes test Loss on drying (110 °C): ≤ 15 % Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
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