GC Analysis of Terpenes in Hops (Cascade Variety) on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber
材料
分析柱
固相微萃取纖維
SPME 纤维组件二乙烯基苯/羧基/聚二甲基硅氧烷 (DVB/CAR/PDMS)
needle size 23 ga, StableFlex, for use with autosampler標準
附屬品
顶空瓶，螺旋盖，圆底（仅小瓶），100个装
volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 ea顶空样品瓶的磁性螺旋盖，18 mm螺纹，1.5mm隔垫，100个装
PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 eaCONDITIONS
sample/matrix
ground Cascade variety hop pellets
SPME fiber
Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)
extraction
20 min, headspace, 40 °C
desorption process
3 min, 270 °C
sample preparation
30 min equilibration at 40 °C prior to extraction
sample preparation
fiber postbake after extraction, 3 min at 270 °C
column
Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U)
oven
60 °C (2 min), 5 °C/min to 275 °C (5 min)
inj. temp.
270 °C
detector
MSD
MSD interface
300 °C
scan range
full scan, m/z 50-500
carrier gas
helium, 1 mL/min constant flow
liner
0.75 mm ID, SPME
说明
分析報告
Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in the Cascade variety of hops. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match. Analysis showed a characteristic terpene profile, with high levels of ß-myrcene, caryophyllene, and humulene present.
其他說明
add 0.5 gm ground hops to a 10 mL headspace vial
法律資訊
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