HPLC Analysis of Sugars and Sugar Enantiomers on Astec^® CYCLOBOND I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

ELSD at 35°C and 3.2 bar nitrogen

injection

10 μL

sample

0.5 mg/mL each in water:acetonitrile, 25:75