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Merck
  • Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids.

Protocol for In Vitro Establishment and Long-Term Culture of Mouse Vaginal Organoids.

STAR protocols (2020-10-29)
Ayesha Ali, Shafiq M Syed, Pradeep S Tanwar
摘要

Healthy vaginal epithelium is essential for normal reproductive functions and protects against infectious diseases. Here, we present a protocol for developing mouse vaginal organoids from single epithelial cells. These organoids recapitulate both functional and structural characteristics of vagina in situ. This model is a powerful tool for investigating how vaginal microbiome or chemicals in contraceptives and personal hygiene products interact with stem cells and alter the epithelial dynamics, which will lead to new insights into the pathogenesis of vaginal diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).

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Roche
DNase I, grade II, from bovine pancreas
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Sigma-Aldrich
台盼蓝 溶液, 0.4%, liquid, sterile-filtered, suitable for cell culture
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Sigma-Aldrich
杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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Sigma-Aldrich
EGF 来源于小鼠, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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