技術
HPLC: suitable
測試參數
column: Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] water; [B] acetonitrile; (25:75, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 10 μL
適合性
application for HPLC
應用
food and beverages
分析報告
This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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