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53583-U

Supelco

Ascentis® Express 90 Å F5 (2.7 μm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

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1 EA
$880.00

$880.00

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1 EA
$880.00

About This Item

UNSPSC代码:
41115700
eCl@ss:
32110501
NACRES:
SB.52

$880.00

请联系客服了解存货情况

产品名称

Ascentis® Express F5, 2.7 μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

物料

stainless steel column

Agency

suitable for USP L43

产品线

Ascentis®

特点

endcapped

制造商/商品名称

Ascentis®

包装

1 ea of

参数

60 °C temp. range
600 bar max. pressure (9000 psi)

技术

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

长度 × 内径

5 cm × 4.6 mm

表面积

135 m2/g

杂质

<5 ppm metals

基质

Fused-Core particle platform
superficially porous particle

基质活性基团

PFP (pentafluorophenyl) phase

粒径

2.7 μm

孔径

90 Å pore size

工作pH值

2-8

应用

food and beverages

分离技术

reversed phase

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相关类别

一般描述

由于存在电负性氟，Ascentis Express F5 的五氟苯基丙基固定相可提供具有缺电子苯环的稳定反相填料。除了形成 pi-pi 和温和的空间相互作用外，F5 相也通过极性相互作用保留化合物。Ascentis Express F5 可用于碱性、酸性或中性化合物，选择性与 C18 不同。

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
USMR001338
USMR001337
USMR001336
USMR001335
USMR001334

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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore

商品

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

实验方案

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns

Fast and Accurate LC-MS Analysis of Vitamin D Metabolites, Using Ascentis® Express F5 HPLC Columns from Supelco®

相关内容

Ascentis® Express F5 (pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Questions

1–10 of 11 Questions  

  1. How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  2. Is there anything special I need to do to my HPLC system to use Ascentis® Express F5 HPLC Columns?

    1 answer

    1. Nothing special is required to use Ascentis® Express F5 HPLC Columns. To obtain the full benefits of Ascentis® Express Columns, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).

      Helpful?

  3. Can I use Ascentis® Express F5 HPLC Columns on any type of HPLC system?

    1 answer

    1. Ascentis® Express F5 HPLC Columns HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  These columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Helpful?

  4. Do I need special fittings and tubing to connect Ascentis® Express F5 HPLC Columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Helpful?

  5. What is the pressure rating for Ascentis® Express F5 HPLC Columns?

    1 answer

    1. Ascentis® Express F5 HPLC Columns are stable to operating pressures up to 600 bar (9000 psi).

      Helpful?

  6. What flow rate should I use with Ascentis® Express F5 HPLC columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis® Express F5 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Helpful?

  7. Are guard columns available for Ascentis® Express F5 4.6 mm ID HPLC Columns?

    1 answer

    1. Yes, we have guard columns available for Ascentis® Express F5 4.6 mm ID HPLC Column.  Product 53500-U is the Ascentis® Express Guard Holder and Product 53599-U Ascentis® Express F5 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 4.6 mm .

      Helpful?

  8. What is the carbon load for Ascentis® Express F5 HPLC Columns?

    1 answer

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis® Express F5 HPLC columns is 3.5 micromoles/meter-squared.

      Helpful?

  9. Can I use Ascentis® Express F5 HPLC Columns on a UHPLC system?

    1 answer

    1. Ascentis® Express F5 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis® Express F5 HPLC Columns outperform sub-2 μm micron columns on many applications since Ascentis® Express F5 HPLC Columns provide the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  10. Can Ascentis® Express F5 HPLC columns be used for LC-MS?

    1 answer

    1. Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis® Express F5 HPLC Columns for high throughput UHPLC and LC-MS are that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Helpful?

1–10 of 11 Questions  

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