推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3F11, monoclonal
recombinant monoclonal
描述
3F11 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt ~40.57 kDa
observed mol wt ~42 kDa
物種活性
mouse, human
物種活性（以同源性預測）
rat
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3F11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Doublecortin. It targets an epitope within 17 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Doublecortin.
應用
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in SH-SY5Y cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Doublecortin in mouse brain tissue sections.
Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected Doublecortin in Neuro-2a and U-87 MG cells.
Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected Doublecortin in frozen mouse brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in SH-SY5Y cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Doublecortin in mouse brain tissue sections.
Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected Doublecortin in Neuro-2a and U-87 MG cells.
Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected Doublecortin in frozen mouse brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Doublecortin, clone 3F11 ZooMAb®, Cat. No. ZRB1863, is a recombinant Rabbit monoclonal antibody that detects Doublecortin and tested for use in Western Blotting, Immunofluorescence, Immunocytochemistry, and Immunohistochemistry (Paraffin).
標靶描述
Neuronal migration protein doublecortin (UniProt: O43602; also known as Doublin,Lissencephalin-X, Lis-X) is encoded by the DCX (also known as DBCN, LISX) gene (Gene ID: 1641) in human. Doublecortin is a microtubule-associated protein that is required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It is expressed almost exclusively in immature neurons. Hence, it has emerged as an important marker of neurogenesis. It is highly expressed in newly produced cells in the neurogenic zones; the sub ventricular zone along the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus. Doublecortin binds to microtubules in migrating cells and as a result promote the movement of these cells. It is reported to direct neuronal migration by regulating the organization and stability of microtubules. Doublecortin may act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein and may participate in a signaling pathway that is crucial for neuronal interaction before and during migration. It undergoes phosphorylation by MARK1, MARK2, and PKA and the phosphorylation regulates its ability to bind microtubules. Doublecortin is reported to be involved in cancer metastasis where unstable interactions between doublecortin and microtubules destabilize cytoskeletal organization leading to disorganized movements of cells that can lead to the uncontrolled migration of cancer cells. Mutations in DCX gene are reported to cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
实验方案
Follow this short antibody reconstitution protocol and see how easy it is to reconstitute ZooMAb® recombinant antibodies.
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