推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
EM00212, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 87.06 kDa
observed mol wt ~105 kDa
物種活性
human
物種活性（以同源性預測）
rat, mouse
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ITGB3(3690)
mouse ... Itgb3(16416)
rat ... Itgb3(29302)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone EM00212 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Integrin β3. It targets an epitope within 41 amino acids from the C-terminal, cytoplasmic domain.
免疫原
GST-tagged recombinant fragment corresponding to 41 amino acids from the C-terminal, cytoplasmic domain of human Integrin beta-3.
應用
Anti-Integrin β3, clone EM00212 ZooMAb, Cat. No. ZRB1515, is a recombinant Rabbit monoclonal antibody that specifically targets Integrin β3 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin β3 in SK-MEL-28, Hs294T, and A375 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin β3 in human placenta and human melanoma tissue sections.
Flow Cytometry Analysis: 1 βg from a representative lot detected Integrin β3 in one million HUVEC.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β3 in HUVEC and SK-MEL-28 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Integrin β3 in human placenta and human melanoma tissue sections.
Flow Cytometry Analysis: 1 βg from a representative lot detected Integrin β3 in one million HUVEC.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin β3 in HUVEC and SK-MEL-28 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Integrin b-3 (UniProt: P05106; also known as Platelet membrane glycoprotein IIIa, GPIIIa, CD61) is encoded by the ITGB3 (also known as GP3A) gene (Gene: 3690) in human. Integrins are heterodimeric cell surface receptors composed of a variable alpha subunit and a conserved beta subunit. The exact combination of various alpha and beta subunits dictates the binding specificity of integrins to different ECM components. Integrins are not constitutively active. Activation of an integrin from low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. Integrin b-3 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 27-718), a transmembrane domain (aa 719-741), and a cytoplasmic domain (aa 742-788). Integrin b-3 heterodimerizes with Integrin alpha-V or with Integrin-alpha IIb to serve as a receptor for fibronectin, vitronectin and other proteins. These heterodimers recognize the sequence RGD in a wide array of ligands. Mutations in ITGB3 gene are known to cause Glanzmann thrombasthenia that is characterized by mucocutaneous bleeding of mild-to-moderate severity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
商品
Learn about the hallmarks of cancer and find reliable ZooMAb® recombinant antibodies to study them, conveniently organized by cancer hallmark.
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