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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1C3, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 242.05 kDa
observed mol wt ~250 kDa
純化經由
using Protein A
物種活性
mouse, human
物種活性（以同源性預測）
rat, monkey, canine, bovine
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
表位序列
Internal
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ARID1A(8289)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1C3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects ARID domain-containing protein 1A (ARID1A). It targets an epitope within 22 amino acids from the internal region. May detect all three isoforms.
免疫原
KLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human ARID domain-containing protein 1A (ARID1A).
應用
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate..
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1A in HeLa cell lysate.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ARID1A in HeLa, A431, HUVEC and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected ARID1A in human colon tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ARID1A with a KD of 4.3 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HeLa cell lysate..
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1A in HeLa cell lysate.
Tested applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ARID1A in HeLa, A431, HUVEC and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected ARID1A in human colon tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound ARID1A with a KD of 4.3 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
AT-rich interactive domain-containing protein 1A (UniProt: O14497; also known as ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BAF250, BRG1-associated factor 250a, BAF250A, Osa homolog 1, hOSA1, SWI-like protein, SWI/SNF complex protein p270, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily F member 1, hELD) is encoded by the ARID1A (also known as BAF250, BAF250A, C1orf4, OSA1, SMARCF1) gene (Gene ID: 8289) in human. ARID1A is a member of a large family of proteins that contain a characteristic ARID domain of approximately 100 amino acids that binds DNA fragments rich in adenine-thymine (AT). It is a component of SWI/SNF chromatin complexes and is highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, and colon. At lower level it is also expressed in several other tissues. It is involved in transcriptional activation and repression of select genes by chromatin remodeling. It changes chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. ARID1A is shown to be critical for embryonic stem cell pluripotency and their differentiation into mesoderm-derived cardiomyocytes and adipocytes. It is also a component of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. Nuclear ARID1A is shown to be more unstable than its cytoplasmic counterpart, as it degrades rapidly, by the ubiquitin-proteasome system of the nucleus. It is normally detected during the G0-G1 phase of the cell cycle and its levels decrease during the G2-S phase. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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