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Merck

T8575

Sigma-Aldrich

抗-色氨酸羟化酶 绵羊抗

affinity isolated antibody, buffered aqueous glycerol solution

别名:

抗-TPH

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

sheep

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

用途

sufficient for 10 blots

物種活性

mammals, human

技術

western blot: 1:1,000 using human dorsal raphe nucleus

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... TPH1(7166)
mouse ... Tph1(21990)
rat ... Tph1(24848)

相关类别

一般說明

色氨酸羟化酶1(TPH1)基因跨越29kbp,具有11个外显子,定位于人染色体11p15.3-p14。TPH1是在肠道、脾脏、胸腺以及松果体和垂体中表达的444个氨基酸蛋白。
色氨酸羟化酶(TPH)是芳香族氨基酸羟化酶(AAAH)家族的成员。

免疫原

重组兔色氨酸羟化酶,从大肠埃希菌中分离为包涵体,并通过制备性SDS-PAGE纯化。

應用

绵羊抗色氨酸羟化酶抗体可用于:
  • 免疫细胞化学
  • 免疫组织化学
  • 免疫荧光
  • 免疫过氧化物酶染色

生化/生理作用

色氨酸羟化酶1基因(TPH1)催化5-羟色氨酸的合成,而5-羟色氨酸是神经递质5-羟色胺的前体。基因表达的变化会影响反应抑制过程中前额叶皮层的激活。TPH1基因的突变会导致精神分裂症,女性肠易激综合征(IBS),自杀行为和抑郁症。编码的蛋白质在调节心血管功能中起着至关重要的作用。

外觀

溶于150 mM NaCl,10 mM HEPES,pH 7.5,100 μg/mL BSA和50%甘油。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Matthew W Hale et al.
Experimental neurology, 224(1), 271-281 (2010-04-13)
Serotonin plays an important role in the regulation of anxiety states and physiological responses to aversive stimuli. Intracerebroventricular (i.c.v.) injection of the stress- and anxiety-related neuropeptide urocortin 2 (Ucn 2) increases c-Fos expression in serotonergic neurons in the dorsal (DRD)
Mahsa Moaddab et al.
Scientific reports, 10(1), 18035-18035 (2020-10-24)
Early adolescent adversity increases adult risk for anxiety disorders. The ventrolateral periaqueductal gray (vlPAG) and neighboring dorsal raphe (DR) are integral to threat prediction, and are responsive to acute stressors. Here, we tested the hypothesis that early adolescent adversity reshapes
Phylogenetic analysis and evolution of aromatic amino acid hydroxylase
Cao J, et al.
Febs Letters, 584(23), 4775-4782 (2010)
Kristina M Wright et al.
eLife, 8 (2019-03-08)
Faced with potential harm, individuals must estimate the probability of threat and initiate an appropriate fear response. In the prevailing view, threat probability estimates are relayed to the ventrolateral periaqueductal gray (vlPAG) to organize fear output. A straightforward prediction is
Leah R Brooks et al.
Behavioural brain research, 264, 74-81 (2014-02-12)
Serotonergic neurons in the dorsal raphe nucleus (DR) are organized in anatomically distinct subregions that form connections with specific brain structures to modulate diverse behaviors, including anxiety-like behavior. It is unclear if the functional heterogeneity of these neurons is coupled

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