T5300

Transforming Growth Factor-β2 来源于猪血小板

≥97% (SDS-PAGE and N-terminal analysis), recombinant, powder, suitable for cell culture

• 别名: TGF-β2
• MDL编号: MFCD00163957
• UNSPSC代码: 12352202
• NACRES: NA.77

属性

• 产品名称: Transforming Growth Factor-β2 来源于猪血小板, powder, suitable for cell culture
• 生物来源: Porcine (Platelets)
• 质量水平: 200
• 方案: ≥97% (SDS-PAGE and N-terminal analysis)
• 表单: powder
• 效能: 0.05-0.5 ng/mL ED₅₀/EC₅₀
• 分子量: protein 25 kDa
• 包装: pkg of 1 μg
• 储存条件: avoid repeated freeze/thaw cycles
• 技术: cell culture | mammalian: suitable
• 杂质: endotoxin, tested
• UniProt登记号: F1S9L0
• 储存温度: −20°C
• 基因信息: pig ... TGFB2(397084)

说明

一般描述

TGF-β2 (transforming growth factor-β2) belongs to the TGF-β superfamily and the gene is mapped to pig (Sus scrofa) chromosome 10p16.[1][2]

生化/生理作用

TGF-β2, like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

TGF-β2 is responsible for maintaining immune homeostasis.[1] TGF-β2 promotes cellular growth, differentiation and survival.[3] TGF-β2 controls lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis.[1] TGF-β2 is a tumor suppress or in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 prevents NK (natural killer) cells growth as well as B and T cell proliferation.[3] TGF-β2 also greatly inhibits the growth of both normal and transformed epithelial. The growth of lymphoid, fibroblast and keratinocyte cells are also known to be hinder by TGF-β2.[4] TGF-β2 controls intestinal homeostasis by functioning in synergy with the bacterial lipopolysaccharide and thereby maintain interleukin-8.[4]

TGF-β2, like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

TGF-β2 belongs to the TGF-β superfamily. TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β2 is a tumor suppress or in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits NK cells growth as well as B and T cell proliferation.

外形

Lyophilized from a 0.2 μm filtered solution in 25% acetonitrile and 0.1% trifluoroacetic acid containing 0.05 mg bovine serum albumin.

分析说明

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

The biological activity of porcine TGF-β2 was tested in culture by measuring its ability to inhibit thymidine incorporation in the IL-4 dependent mouse T-helper cell line HT-2. The EC50 is defined as the effective concentration of growth factor that elicits 50% inhibition of cell growth in a cell based bioassay.

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable
• 个人防护装备: Eyeshields, Gloves, type N95 (US)