推荐产品
product name
破伤风毒素 来源于破伤风梭菌,
分子量
150 kDa (heavy chain (100 kDa) and a light chain (50 kDa) connected through an interchain disulfide bond)
品質等級
溶解度
H2O: soluble
儲存溫度
2-8°C
基因資訊
Clostridium tetani E88 ... tetX(1061100)
一般說明
Tetanus toxin is also known as tetanospasmin. It is a 150-kDa protein, produced by Clostridium tetani. This protein consists of a light and a heavy chain.
應用
Tetanus toxin from Clostridium tetani has been used as the exocytosis inhibitor/Golgi-dependent secretion to examine whether secretory carrier membrane protein (SCAMP5) stimulated α-synuclein secretion via the conventional Golgi-dependent pathway. It has also been used in peripheral blood mononuclear cells (PBMCs) culture for T cell proliferation screening.
Tetanus toxin from Clostridium tetani has been used to inhibit vesicular release in Pannexin 1-transfected Neuro2A cells1.
生化／生理作用
Binds to polysialogangliosides acceptors on surface of all peripheral presynaptic nerve terminals. Internalized by receptor-mediated endocytosis and carried to spinal cord and brain by transsynaptic retrograde transport. Blocks the release of glycine from inhibitory interneurons of the spinal cord. Cleaves synaptobrevin (VAMP-2), and blocks synaptic vesicle exocytosis in vivo.
Tetanus toxin can cause spastic paralysis.
準備報告
Purified from cultures of Clostridium tetani by a modification of the method of Robinson, et al.
重構
Each vial, when reconstituted with 100 μL of sterile purified water, contains 25 μg of tetanus toxin in 20 mM HEPES, pH 7.4 with 1.25% lactose.
分析報告
Two bands by SDS-PAGE under reducing conditions (100 kDa heavy chain and 50 kDa light chain).
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 1 Oral - Acute Tox. 2 Inhalation - STOT SE 1
標靶器官
Central nervous system
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
MODELS vert The Tetanus Toxin Model of Temporal Lobe Epilepsy
Endocrine regulations, 804-807 (2009)
Felix Horns et al.
Cell reports, 30(3), 905-913 (2020-01-23)
Antibody memory protects humans from many diseases. Protective antibody memory responses require activation of transcriptional programs, cell proliferation, and production of antigen-specific antibodies, but how these aspects of the response are coordinated is poorly understood. We profile the molecular and
Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of alpha-synuclein via exosome
Yang Y, et al.
PLoS ONE, 12(7), e0180892-e0180892 (2017)
Tetanus
Hunter's Tropical Medicine and Emerging Infectious Disease, 508-510 (2013)
Rodolfo M Iglesias et al.
Neurochemical research, 37(6), 1355-1363 (2012-02-24)
Pannexin1 (Panx1), a protein related to the gap junction proteins of invertebrates, forms nonjunctional channels that open upon depolarization and in response to mechanical stretch and purinergic receptor stimulation. Importantly, ATP can be released through Panx1 channels, providing a possible
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