价格与库存信息目前不能提供
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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
BC-24, monoclonal
包含
15 mM sodium azide
种属反应性
human
技术
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using protease-digested sections of adult human tissue.
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
radioimmunoassay: suitable
western blot: suitable
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... TNN(63923)
一般描述
Monoclonal Anti-Human Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Tenascin (TN) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. Human TN has 3 subunits of 190, 200 and 220 kD. TN has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. The expression of TN is associated with development and growth, both normal and pathological, with restricted distribution in normal adult tissue. It is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells. Human and chicken TN contain an RGD sequence which may function in cell adhesion and it seems likely that TN mediates cell attachment through an RGD-dependent integrin receptor. Mouse TN does not contain an RGD sequence in the third type III repeat implicated in cell attachment, or in any other positions.
特异性
The antibody recognizes an epitope within the N-terminal EGF-like (EGF-L) sequence of the molecule. It recognizes all human tenascin isoforms.
免疫原
human tenascin.
应用
Monoclonal Anti-Tenascin antibody produced in mouse has been used for immunohistochemistry.
Mouse monoclonal clone BC-24 anti-Tenascin antibody is an essential tool for the localization, identification and studies on the role of tenascin in epithelial-mesenchymal and neuronal-glial interactions.
生化/生理作用
Tenascin (TN) is up-regulated by epithelial transforming growth factor β (TGF-β) in mesenchyme. TN in the stroma of cancerous tissue contributes to tumorigenesis and progression of metastasis. High molecular weight TN isoform, a stromal marker of breast cancer, over-expresses implicating cell proliferation and invasion. TN has a potential role in tissue restoration. Intracytoplasmic TN immunoreactivity has been detected in malignant melanomas and in lung carcinomas. TN expression, when reacted with monoclonal antibody, determines its localization in tumor cells and its association with epithelial-mesenchymal and neuronal-glial cell interactions.
外形
This product is supplied as ascites fluid containing 15 mM sodium azide
储存及稳定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Tenascin-C in primary malignant melanoma of the skin
Ilmonen S, et al.
Histopathology, 45(4), 405-411 (2004)
G I Mason et al.
Annals of the rheumatic diseases, 59(3), 183-189 (2000-03-04)
To determine whether there was altered elaboration of non-collagenous extracellular matrix proteins or expression of TGFbeta isoforms and their receptors in salivary glands of patients with systemic sclerosis (SSc) and Raynaud's phenomenon (RP). Because of the possible role of mast
Changes in tenascin-C isoform expression in invasive and preinvasive breast disease
AdamS M, et al.
Cancer Research, 62(11), 3289-3297 (2002)
Naoko Yoneura et al.
International journal of molecular medicine, 42(2), 821-830 (2018-05-12)
The interaction between cancer cells and stromal components contributes to cancer invasion and metastasis in pancreatic ductal adenocarcinoma (PDAC). The present study investigated the role of the correlation between annexin II (ANX2) and stromal tenascin C (TNC) with the progression of PDAC.
Allegra G Hawkins et al.
Molecular & cellular proteomics : MCP, 17(5), 901-912 (2018-02-02)
Tumor: tumor microenvironment (TME) interactions are critical for tumor progression and the composition and structure of the local extracellular matrix (ECM) are key determinants of tumor metastasis. We recently reported that activation of Wnt/beta-catenin signaling in Ewing sarcoma cells induces
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