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生物源
human
重組細胞
expressed in HEK 293 cells
籤條
6-His tagged (C-terminus)
化驗
≥98% (SDS-PAGE)
形狀
lyophilized
分子量
calculated mol wt 27.6 kDa
observed mol wt 40 kDa by SDS-PAGE (reducing) (Ser 21 is the predicted N-terminal .)
包裝
pkg of 10 μg
雜質
<1 EU/μg endotoxin (LAL test)
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... RSPO1(284654)
一般說明
R-spondin-1, also known as Roof plate-specific Spondin 1 (RSPO1) and cysteine rich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. All R spondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteine rich furin like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin like domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively.
應用
R spondin-1 human has been used to study the mechanism of action of methylphenidate (a drug to treat attention-deficit hyperactivity disorder), its effect on cell proliferation and maturation.
生化／生理作用
RSPOs (roof plate-specific spondin) plays a crucial role in embryogenesis and tissue homeostasis. During the developmental stages of ovary, RSPO1 becomes a necessary factor for sex determination and follicular cell progenitor differentiation. The RSPO1 gene expression is also predicted to initiate ovarian cancer. It has a supportive role in cell proliferation, differentiation, and survival. RSPO1 is centrally associated with intestinal stem cell induction.
外觀
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
重構
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
Methylphenidate enhances neuronal differentiation and reduces proliferation concomitant to activation of Wnt signal transduction pathways.
Grunblatt E, et al.
Translational Psychiatry, 8(1), 51-51 (2018)
R-Spondin1 regulates Wnt signaling by inhibiting internalization of LRP6.
Binnerts M E, et al.
Proceedings of the National Academy of Sciences of the USA, 104(37), 14700-14705 (2007)
Multiplicity of the interactions of Wnt proteins and their receptors.
Kikuchi A, et al.
Cellular Signalling, 19(4), 659-671 (2007)
RSPOs facilitated HSC activation and promoted hepatic fibrogenesis.
Yin X, et al.
Oncotarget, 7(39), 63767-63767 (2016)
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