SRP4835

PCNA human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE), ≥90% (HPLC)

• 别名: Cyclin, MGC8367, PCNA, Proliferating cell nuclear antigen
• 分類程式碼代碼: 12352202
• NACRES: NA.32

属性

• 生物源: human
• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: ≥90% (HPLC); ≥90% (SDS-PAGE)
• 形狀: liquid
• 分子量: ~29.8 kDa
• 包裝: pkg of 5 μg
• 雜質: endotoxin, tested
• NCBI登錄號: P12004
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PCNA(5111)

说明

一般說明

PCNA (proliferating cell nuclear antigen) gene is mapped to human chromosome 20p12.3 and encodes a scaffolding protein. PCNA is a homotrimeric protein known to localize in the nucleus. The PCNA ring has a central cavity that is large enough to allow the dsDNA to slide freely along it.

生化／生理作用

Proliferating cell nuclear antigen (PCNA) has its main function in DNA replication and repair mechanism. Itsignificantly maintains the longevity of mature neutrophil granulocytes. Upregulation of the PCNA gene is observed in cervical squamous cell carcinoma. PCNA supports cell survival by elevating metabolism flux. This gene expression is known to offer resistance to chemotherapy. Expression of PCNA or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.

外觀

Sterile filtered; 5 μg/5 μl in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.

重構

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.