推荐产品
一般說明
The sonic hedgehog protein is encoded by the SHH gene that is mapped to human chromosome 7q36. It is homologous to the Drosophila segment polarity gene hedgehog. The encoded protein is a 45kDa precursor protein that is auto-cleaved to form SSH-N (an amino-terminal 20kDa product) and SSH-C (carboxy-terminal product). SSH-N is found to be cell-associated, while SSH-C diffuses freely. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring chemically modified Cys residue.
應用
Sonic Hedgehog human has been used:
- To study its role in liver cancer stem cell activity.
- For neuronal differentiation.
- For cholinergic-like neurons (ChLNs) differentiation.
The sonic hedgehog protein is used in neuronal induction medium as a supplement.
生化／生理作用
The SHH (sonic hedgehog) gene encodes a soluble signaling protein that functions in the patterning of cell differentiation in the neural tube and limb bud. It has also been implicated in several processes, such as developing nervous system, including midbrain and ventral forebrain neuronal differentiation and neuronal precursor proliferation. In adults, it regulates progenitor function and proliferation. Mutations in this gene have been associated with holoprosencephaly, a developmental defect of the forebrain and midface.
序列
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
外觀
Lyophilized with no additives.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Mutations in the human Sonic Hedgehog gene cause holoprosencephaly.
Roessler E
Nature Genetics, 14, 357-360 (1996)
Use of human induced pluripotent stem cell-derived neurons as a model for cerebral toxoplasmosis
Tanaka N, et al.
Microbes and Infection, 18(7-8), 496-504 (2016)
Use of human induced pluripotent stem cell-derived neurons as a model for Cerebral Toxoplasmosis.
Tanaka N
Microbes and Infection, 18, 496-504 (2016)
Direct transdifferentiation of human Wharton?s Jelly mesenchymal stromal cells into Cholinergic-like neurons
Mendivil-Perez M, et al.
Journal of Neuroscience Methods (2018)
The SHH/Gli axis regulates CD 90-mediated liver cancer stem cell function by activating the IL 6/JAK 2 pathway
Zhang K, et al.
Journal of Cellular and Molecular Medicine, 22(7), 3679-3690 (2018)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门