After reconstituting the protein in water, it remains active and stable at 2-8°C for up to a week. However, for long-term stabilization at -20 to -80°C, further dilution with a buffer containing a carrier is necessary to prevent activity loss. It is recommended to use 5% trehalose or 0.1% BSA in water or PBS for extended storage. Working aliquots should have a minimum volume of 10 uL and a concentration above 1 ug/ml for optimal stability.
推荐产品
相关类别
一般描述
应用
EGF（表皮生长因子）人已经在培养基中用于维持 MCF10A细胞（人乳腺上皮细胞）。[2]
生化/生理作用
序列
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
外形
冻干，无添加剂。
重悬
开盖前先离心试剂管。 在水中重悬至浓度为0.1-1.0mg/ml。 不要涡旋。 该溶液可在2-8℃ 下储存，长达 1 周。 要长期储存，建议在含有稳定剂（例如5％ 海藻糖）的缓冲液中进一步稀释，并在-20°C至-80°C，以工作等分试样储存。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.
Overton KW
Proceedings of the National Academy of Sciences of the USA, 111, E4386-E4393 (2014)
The miR-200 family regulates the epithelial-mesenchymal transition induced by EGF/EGFR in anaplastic thyroid cancer cells.
Zhang Z
International Journal of Molecular Medicine, 30, 856-862 (2012)
Clinical Implication of EGF A61G Polymorphism in the Risk of Non Small Cell Lung Adenocarcinoma Patients: A Case Control Study.
Masroor M
Asian Pacific Journal of Cancer Prevention, 16, 7529-7534 (2015)
Neurotrophic actions of a novel molluscan epidermal growth factor.
Hermann PM
The Journal of Neuroscience, 20, 6355-6364 (2000)
Reactive oxygen species (ROS) are essential mediators in epidermal growth factor (EGF)-stimulated corneal epithelial cell proliferation, adhesion, migration, and wound healing.
Huo Y
Experimental Eye Research, 89, 876-886 (2009)
商品
Discover answers to your frequently asked questions about animal component-free, xenobiotic-free, and chemically defined media and reagents for cell culture.
-
Is it necessary to dilute item no: SRP3027-500UG in a buffer with a stabilizer for extended storage as mentioned in the reconstitution portion of the specification. If so, would D-(+)-Trehalose dihydrate (cat#T0167) be appropriate? Also, specify the buffer considering that water is indicated as an appropriate diluent in the specifications.
1 answer-
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持
