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Merck
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主要文件

SRP0130

Sigma-Aldrich

EZH1/EED/SUZ12  human

recombinant, expressed in baculovirus infected insect cells

别名:

ENX-2, EZH1, Enhancer of zeste homolog 1, KMT6B, Lysine N-methyltransferase 6B

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32
价格与库存信息目前不能提供

生物来源

human

重组

expressed in baculovirus infected insect cells

方案

≥80% (SDS-PAGE)

表单

aqueous solution

分子量

51 kDa (EED)
86 kDa (EZH1)
87 kDa (SUZ12)

包装

pkg of 50 μg

浓度

>0.02 mg/mL

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... EZH1(2145)

一般描述

Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12,. NM_015355, amino acids 2-end, with N-terminal His tag.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

历史批次信息供参考:

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