As per the product information sheet, 100% Ethanol is suggested for the dilution of the wash solution. The use of 100% methanol has not been validated and is not recommended for the isolation of RNA. Kindly review the product information sheet available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/188/017/snc10bul.pdf
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一般描述
Sigma mirPremier microRNA分离试剂盒可以快速、有效地从不同的生物学来源样本，包括哺乳动物细胞培养物、动物组织、植物组织、微生物培养物中纯化和富集miRNA和其他小分子RNA，且无需进行任何有害的有机萃取。microRNA (miRNA) 属于小分子RNA，长度大约为21个核苷酸(nt)，通过各种方式调节着基因表达，包括翻译抑制、mRNA切割和脱腺苷化。此外，如果您关注信使RNA和其它大分子RNA，这种试剂盒还可用于分离总RNA。
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应用
特点和优势
- 旨在提高各种生物学来源的miRNA和其它小RNA分子的分离效率
- 在30分钟内为下游应用实现快速而高效的miRNA提取和浓缩
- 可以提取不含大RNA的高纯度miRNA
- 不涉及危险的有机提取
法律信息
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Warning
危险分类
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is
Rong-Hua Lu et al.
Fish physiology and biochemistry, 46(5), 1665-1677 (2020-05-25)
Hepatic lipid metabolism disorder due to excessive fat accumulation in fish is a significant problem in aquaculture. Studies have shown that grape seed procyanidin extract (GSPE) can regulate fish lipid metabolism and improve fish immunity. However, the mechanism is unclear.
Unicellular origin of the animal microRNA machinery
Br?te J, et al.
Current Biology, 28(20), 3288- 3295 (2018)
商品
简单的DNA和RNA纯法方法大幅推动了基因组和基因表达的分析和鉴定。人们需要快速、方便地从多种细胞来源分离DNA和RNA，包括哺乳动物、植物和细菌培养物来源的细胞和组织。
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
相关内容
Sigma-Aldrich® Advanced Genomics是CRISPR、Cas9、合成向导RNA(sgRNA)和锌指核酸酶(ZFN)等基因编辑和沉默技术的领先提供者。
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
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Methanol 100% can be used for the dilution of the wash solution instead of ethanol 100%?
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
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Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
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Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
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We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
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We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
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