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Merck

SHM01

Sigma-Aldrich

ExpressMag® Super Magnetic Kit

Increases transduction efficiency

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About This Item

分類程式碼代碼:
41106609

產品線

MISSION®

儲存期限

≥2 yr at ~4 °C

儲存溫度

2-8°C

一般說明

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

應用

MISSION® ExpressMag® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.

注意

Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.
Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

法律資訊

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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产品编号
说明
化学品安全说明书

  • SHM03MISSION® ExpressMag® Beads, Increases transduction efficiency化学品安全说明书

  • SHM04MISSION® ExpressMag® Super Magnetic Plate, Increases transduction efficiency化学品安全说明书

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。

实验方案

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

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