SEP020 is designed to deplete the top 14 abundant human proteins, while PROT20LC is designed to deplete the top 20 abundant human proteins. Also, all of the antibodies in SEP020 are IgY antibodies, while the PROT20LC antibodies and antibody ligands are primarily IgG-based.
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柱直徑 × 長度
6.4 mm × 63.0 mm
容量
2.5 mg total protein loading(35 μL of human plasma, average human protein concentration 70 mg/mL)
儲存溫度
2-8°C
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一般說明
Seppro® IgY14 柱可从血浆蛋白混合物中去除14种高丰度蛋白。与同类产品相比，鸡的多克隆IgY（蛋黄免疫球蛋白）抗体具有显著的高特异性分离优势，可为研究者提供更干净的人蛋白质样品。Seppro® 柱帮助研究人员更加简单的完成对血浆蛋白质组的进一步蛋白质组学分析，并且获得更为清晰的结果。
可去除的蛋白质：
白蛋白
IgG
α1-抗胰酶蛋白
IgA
IgM
转铁蛋白
触珠蛋白
α2-巨球蛋白
纤维蛋白原
补体蛋白C3
α1-酸性糖蛋白 (血清类粘蛋白)
HDL (载脂蛋白A-I 和A-II)
LDL (主要是载脂蛋白B)
可去除的蛋白质：
白蛋白
IgG
α1-抗胰酶蛋白
IgA
IgM
转铁蛋白
触珠蛋白
α2-巨球蛋白
纤维蛋白原
补体蛋白C3
α1-酸性糖蛋白 (血清类粘蛋白)
HDL (载脂蛋白A-I 和A-II)
LDL (主要是载脂蛋白B)
当粗生物学样品通过色谱柱时，固定的特异性IgY会同时去除靶向高丰度的蛋白质。选择性免疫耗竭为下游蛋白质组学分析提供了丰富的低丰度蛋白库。对这14种高丰度蛋白质的特异性去除使人血清或血浆中的蛋白质总量减少了约95％。然后，可以对流出部分中的低丰度蛋白质进行研究。去除高丰度蛋白质可提高一维电泳（1DGE）、二维（2DGE）电泳和液相色谱/质谱（LC/MS）的分辨率和动态范围。根据下游应用，可能需要对收集的流出部分进行浓缩。
應用
Seppro® IgY14，LC2（2 mL柱床体积柱）基于免疫亲和作用，是专门的血浆/血清蛋白去除工具，可通过液相色谱法去除人血浆蛋白混合物中的14种高丰度蛋白(HAP)。Seppro® IgY14 完美支持对人血清/血浆蛋白中低丰度蛋白的分析。
儲存和穩定性
将色谱柱保存在2–8°C。使用后，用含0.02％叠氮化钠的1´Dilution Buffer平衡色谱柱，并将色谱柱在2-8°C下保存，并密封好端盖。请勿冷冻色谱柱。
其他說明
如果样品的蛋白质浓度未知，并且血清总蛋白水平可能升高，建议将血清负载量降至24.0 ml，进行初步研究，以避免潜在的大量蛋白流失。
法律資訊
Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
Effect of plasma protein depletion on BNP-32 recovery.
Adam M Hawkridge et al.
Clinical chemistry, 54(5), 933-934 (2008-04-30)
Does plasma protein depletion increase lung liquid conductance?
P Dodek
American journal of respiratory and critical care medicine, 154(4 Pt 1), 1213-1215 (1996-10-01)
Renato Millioni et al.
PloS one, 6(5), e19603-e19603 (2011-05-17)
To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number
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How would I decide between Products SEP020, Seppro® IgY, and PROT20LC, ProteoPrep® 20 Plasma Immunodepletion LC Columns?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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My protein sample contains SDS. Is this compatible with Product SEP020, Seppro® IgY14?
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No. We do not recommend using protein samples containing SDS with Product No. SEP020. SDS is a detergent and would be expected to affect adversely the activity of the conjugated antibody in terms of depletion.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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