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Merck

SAE0214

Sigma-Aldrich

Leucoagglutinin from P. vulgaris, recombinant

Expressed in P. pastoris, lyophilized powder, suitable for cell culture

别名:

PHA-L, Phytohemagglutinin-L, Red Kidney Bean Lectin

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.21

品質等級

形狀

lyophilized powder

UniProt登錄號

運輸包裝

wet ice

儲存溫度

-10 to -25°C

一般說明

Lectin from Red Kidney Bean has long been used in a wide variety of applications that require carbohydrate binding including but not limited to T-cell proliferation, leucoagglutination, glycoconjugate capture and more. The native form of lectin from Red Kidney Bean is composed of 2 isolectins (PHA-L and PHA-E) which combine in heterotetromeric combinations that elicit a variety of properties. This recombinant form is strictly the leucoagglutinin (PHA-L) isolectin only, so the erythroagglutinin (PHA-E) isolectin is absent in this product. This allows for more selective performance because there is no chance of PHA-E contamination as seen in native source products.

生化/生理作用

Kidney bean lectin has a history of being used as a phytohemagglutinin because of its ability to agglutinate blood cells. This isolectin (PHA-L) specficially is the Leucoagglutinin type and can be used for leucoagglutination and as a mitogen. As a lectin, it is known to have some selectivity for binding of β1-6 branched N-glycans.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities
Daniel Bojar
ACS Chemical Biology, 17(11), 2993-3012 (2022)

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