推荐产品
一般說明
Filipin III是一种多烯大环内酯类抗生素，是从S. filipinensis培养物中分离出来的主要成分。1 Filipin因其能够发出荧光并与胆固醇结合的能力而被广泛
用作生物膜中甾醇定位的探针 2，以及在C型Niemann-Pick病的研究中，在临床上用作游离胆固醇的染色剂。 3 Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。 4 Filipin III被发现可在烟草细胞中引发信号反应，包括NADPH氧化酶依赖性产生。5。其抗真菌作用机制可能是通过与膜甾醇结合而改变膜通透性和相关功能。Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。
用作生物膜中甾醇定位的探针 2，以及在C型Niemann-Pick病的研究中，在临床上用作游离胆固醇的染色剂。 3 Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。 4 Filipin III被发现可在烟草细胞中引发信号反应，包括NADPH氧化酶依赖性产生。5。其抗真菌作用机制可能是通过与膜甾醇结合而改变膜通透性和相关功能。Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。
應用
Filipin已在双染色操作中作为探针，用于检测聚丙烯酰胺凝胶中的脂蛋白并将其固定在硝酸纤维素膜上。它还广泛用于通过特定的荧光复合物对未酯化的胆固醇进行定位和定量。
其他說明
- Filipin III溶液对空气和光非常敏感。收到产品后请进行等分并在-20°C条件下储存，避免循环冻融。
- Filipin III可多种浓度进行使用，其取决于所使用的具体方案。根据所使用的方案，可用合适的缓冲液对1mg/ml的储备溶液进行稀释。
- Filipin III与胆固醇的相互作用改变了吸收和荧光光谱，因此在用荧光显微镜观察时，使用340-380nm作为激发波长，385-470nm作为发射波长（Filipin荧光染色光漂白速度非常快，因此应立即对样品进行分析） 。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
188.6 °F
閃點（°C）
87 °C
其他客户在看
Wen-Bin Zhang et al.
Acta neuropathologica communications, 11(1), 102-102 (2023-06-22)
Accumulating evidences reveal that cellular cholesterol deficiency could trigger the onset of Alzheimer's disease (AD). As a key regulator, 24-dehydrocholesterol reductase (DHCR24) controls cellular cholesterol homeostasis, which was found to be downregulated in AD vulnerable regions and involved in AD-related
Carla E Cadena Del Castillo et al.
Nature communications, 12(1), 4898-4898 (2021-08-14)
Hedgehog (Hh) signaling is essential during development and in organ physiology. In the canonical pathway, Hh binding to Patched (PTCH) relieves the inhibition of Smoothened (SMO). Yet, PTCH may also perform SMO-independent functions. While the PTCH homolog PTC-3 is essential
Yun-Qi Zhou et al.
Emerging microbes & infections, 11(1), 1135-1144 (2022-03-29)
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants is threatening public health around the world. Endocytosis functions as an important way for viral infection, and SARS-CoV-2 bears no exception. However, the specific endocytic mechanism of
Pan Gao et al.
Investigative ophthalmology & visual science, 63(5), 32-32 (2022-05-27)
Bietti crystalline dystrophy (BCD) is a progressive retinal degenerative disease primarily characterized by numerous crystal-like deposits and degeneration of retinal pigment epithelium (RPE) and photoreceptor cells. CYP4V2 (cytochrome P450 family 4 subfamily V member 2) is currently the only disease-causing
Nina H Pipalia et al.
Journal of lipid research, 47(2), 284-301 (2005-11-17)
Niemann-Pick disease type C (NPC) is an autosomal recessive genetic disorder manifested by abnormal accumulation of unesterified cholesterol and other lipids. We screened combinatorially synthesized chemical libraries to identify compounds that would partially revert cholesterol accumulation. Cultured CHO cells with
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门