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生物源
alpaca
重組細胞
expressed in E. coli
共軛
biotin
抗體表格
affinity purified immunoglobulin
抗體產品種類
secondary antibodies
無性繁殖
recombinant monoclonal
形狀
lyophilized solid
分子量
14 kDa
物種活性
Alpaca, mouse
技術
ELISA: 1:5000-1:20000
western blot: 1:5000-1:20000
尺寸
0.1 mL
顏色
colorless
同型
VHH
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
Alpaca Anti-Mouse kappa Biotin single domain Antibody is designed to specifically detect mouse IgG kappa chain. It is a monoclonal, single domain, recombinant antibody to mouse IgG kappa chain coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse kappa Biotin single domain Antibody reacts with the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特異性
Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c
Isotype: VHH domain of alpaca IgG2b/2c
免疫原
Recombinant Alpaca single variable domain secondary nano antibody
包裝
100 μg in Glass bottle
外觀
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
重構
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
儲存和穩定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
其他說明
No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
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訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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