SAB5702871

Anti-IDH1 Antibody, clone 5S2U7, Rabbit Monoclonal

• 别名: HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
• UNSPSC代码: 12352203
• NACRES: NA.46

属性

• 生物来源: rabbit
• 质量水平: 100
• 物料: colorless
• 克隆: 5S2U7, monoclonal
• 表单: liquid
• 分子量: 45 kDa
• 种属反应性: rat, human, rat
• 浓度: 1.36 mg/mL
• 技术: immunofluorescence: 1:50 - 1:200; immunohistochemistry: 1:50 - 1:200; immunoprecipitation (IP): 1:50 - 1:200; western blot: 1:500 - 1:2000
• 颜色: colorless
• 同位素/亚型: IgG
• 免疫原序列: DATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPG
• UniProt登记号: O75874
• 运输: wet ice
• 储存温度: −20°C
• 靶向翻译后修饰: unmodified
• 基因信息: human ... IDH1(3417)

说明

一般描述

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]

免疫原

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IDH1 (NP_005887.2).

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.

应用

WB, IHC, IF/ICC, IP

安全信息

• 象形图: GHS07,GHS09
• 警示用语：: Warning
• 危险声明: H317,H411
• 预防措施声明: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• 危险分类: Aquatic Chronic 2 - Skin Sens. 1
• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 3